http://oar.icrisat.org/id/eprint/10043 2017-06-12T03:46:07Z info:eu-repo/semantics/descriptiveMetadata http://oar.icrisat.org/10043/ Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea Saxena, R K Singh, V K Kale, S M Tathineni, R Parupalli, S Kumar, V Garg, V Das, R R Sharma, M Yamini, K N Muniswamy, S Ghanta, A Rathore, A Sameer Kumar, C V Saxena, K B Kishor, P B K Varshney, R K Pigeonpea Genetics and Genomics Fusarium wilt (FW) is one of the most important biotic stresses causing yield losses in pigeonpea. Genetic improvement of pigeonpea through genomics-assisted breeding (GAB) is an economically feasible option for the development of high yielding FW resistant genotypes. In this context, two recombinant inbred lines (RILs) (ICPB 2049 × ICPL 99050 designated as PRIL_A and ICPL 20096 × ICPL 332 designated as PRIL_B) and one F2 (ICPL 85063 × ICPL 87119) populations were used for the development of high density genetic maps. Genotyping-by-sequencing (GBS) approach was used to identify and genotype SNPs in three mapping populations. As a result, three high density genetic maps with 964, 1101 and 557 SNPs with an average marker distance of 1.16, 0.84 and 2.60 cM were developed in PRIL_A, PRIL_B and F2, respectively. Based on the multi-location and multi-year phenotypic data of FW resistance a total of 14 quantitative trait loci (QTLs) including six major QTLs explaining >10% phenotypic variance explained (PVE) were identified. Comparative analysis across the populations has revealed three important QTLs (qFW11.1, qFW11.2 and qFW11.3) with upto 56.45% PVE for FW resistance. This is the first report of QTL mapping for FW resistance in pigeonpea and identified genomic region could be utilized in GAB. Nature Publishing Group 2017-05-15 Article PeerReviewed application/pdf en http://oar.icrisat.org/10043/1/41598_2017_Article_1537.pdf Saxena, R K and Singh, V K and Kale, S M and Tathineni, R and Parupalli, S and Kumar, V and Garg, V and Das, R R and Sharma, M and Yamini, K N and Muniswamy, S and Ghanta, A and Rathore, A and Sameer Kumar, C V and Saxena, K B and Kishor, P B K and Varshney, R K (2017) Construction of genotyping-by-sequencing based high-density genetic maps and QTL mapping for fusarium wilt resistance in pigeonpea. Scientific Reports, 7 (1911). pp. 1-11. ISSN 2045-2322 http://dx.doi.org/10.1038/s41598-017-01537-2 10.1038/s41598-017-01537-2 info:eu-repo/semantics/objectFile info:eu-repo/semantics/humanStartPage