@article{icrisat10027, month = {June}, title = {Effect of biosynthesized Silver nanoparticles on growth and development of Helicoverpa armigera (Lepidoptera: Noctuidae): Interaction with midgut protease}, publisher = {Elsevier}, year = {2017}, author = {S Kantrao and M A Ravindra and S M D Akbar and P D Kamala Jayanthi and A Venkataraman}, pages = {583--589}, volume = {20}, journal = {Journal of Asia-Pacific Entomology}, number = {2}, keywords = {Helicoverpa armigera; Biosynthesis; Silver nanoparticles; Gut protease; Tryptophan fluorescence; Absorbance}, url = {http://oar.icrisat.org/10027/}, abstract = {We investigated the effect of Silver nanoparticles (AgNPs) on the gut protease activity of insecticide resistant gram caterpillar, Helicoverpa armigera. The leaf extracts of Peepal tree, Ficus religiosa (FR) and banyan tree, Ficus benghalensis (FB) mediated biogenic AgNPs were synthesized to modulate the function of gut protease activity in H. armigera (Ha). Bioassay with FR and FB AgNPs significantly reduced both larval weight and survival rate of H. armigera. The FR and FB AgNPs inhibited the Ha-Gut protease activity by 50 and 70\% at 100 {\ensuremath{\mu}}g concentration respectively. The FR and FB Silver nanoparticles were interacted and binds with high affinity with protease. The inhibition studies on Ha-gut protease activity may contribute towards developing new IRM (Insecticide Resistant Management) strategies against H. armigera to overcome insecticidal resistance issues.} }