Root aquaporins contribute to whole plant water fluxes under drought stress in rice (Oryza sativaL.)

Grondin, A and Mauleon, R and Vadez, V and Henry, A (2016) Root aquaporins contribute to whole plant water fluxes under drought stress in rice (Oryza sativaL.). Plant, Cell & Environment, 39 (2). pp. 347-365. ISSN 01407791

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Abstract

Aquaporin activity and root anatomy may affect root hydraulic properties under drought stress. To better understand the function of aquaporins in rice root water fluxes under drought, we studied the root hydraulic conductivity (Lpr) and root sap exudation rate (Sr) in the presence or absence of an aquaporin inhibitor (azide) under well-watered conditions and following drought stress in six diverse rice varieties. Varieties varied in Lpr and Sr under both conditions. The contribution of aquaporins to Lpr was generally high (up to 79% under well-watered conditions and 85% under drought stress) and differentially regulated under drought. Aquaporin contribution to Sr increased in most varieties after drought, suggesting a crucial role for aquaporins in osmotic water fluxes during drought and recovery. Furthermore, root plasma membrane aquaporin (PIP) expression and root anatomical properties were correlated with hydraulic traits. Three chromosome regions highly correlated with hydraulic traits of the OryzaSNP panel were identified, but did not co-locate with known aquaporins. These results therefore highlight the importance of aquaporins in the rice root radial water pathway, but emphasize the complex range of additional mechanisms related to root water fluxes and drought response.

Item Type: Article
Divisions: Research Program : Innovation Systems for the Drylands (ISD)
CRP: UNSPECIFIED
Uncontrolled Keywords: aquaporins; plasma membrane intrinsic proteins; azide; root hydraulic conductivity; sap exudation; leaf water potential; transpiration rate; rice
Subjects: Others > Rice
Depositing User: Mr Ramesh K
Date Deposited: 01 May 2017 10:53
Last Modified: 01 May 2017 10:53
URI: http://oar.icrisat.org/id/eprint/9981
Official URL: http://dx.doi.org/10.1111/pce.12616
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This work was funded by the Generation Challenge Programme project G3008.06 ‘Targeting Drought-Avoidance Root Traits to Enhance Rice Productivity under Water-Limited Environments’. We thank D. Morales, L. Satioquia, A. Reyes, E. Mateo, P. Zambrano, A. Oxina, A. Los Años, E. Mico, M. Natividad, N. Sadiasa, M. Quintana, N. Turingan, C. L. Cabral and R. Torres from the IRRI Drought physiology group for technical support. At IRRI, we are also grateful to Dr. I. H. Slamet-Loedin, Dr. N. Tsakirpaloglou and Dr. K. R. Trijatmiko for their support and advice on the RT-PCR experiment, to J. Detras for support with the Map Chart software, to V. Juanillas for support on IRRI-GSL-Galaxy analysis, to V. Bartolome for statistical support and to Dr. A. Ismail for helpful comments on the manuscript. At ICRISAT, we thank Dr. J. Kholova, S. Mallayee and M. Anjaiah for useful discussions and help in transpiration measurements, and A. C. Pandey for assistance in plant culture.
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