Genetic Dissection of Novel QTLs for Resistance to Leaf Spots and Tomato Spotted Wilt Virus in Peanut (Arachis hypogaea L.)

Pandey, M K and Wang,, H and Khera, P and Vishwakarma, M K and Kale, S M and Culbreath, A K and Holbrook, C C and Wang, X and Varshney, R K and Guo, B (2017) Genetic Dissection of Novel QTLs for Resistance to Leaf Spots and Tomato Spotted Wilt Virus in Peanut (Arachis hypogaea L.). Frontiers in Plant Science, 8 (25). pp. 1-12. ISSN 1664-462X

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Abstract

Peanut is an important crop, economically and nutritiously, but high production cost is a serious challenge to peanut farmers as exemplified by chemical spray to control foliar diseases such as leaf spots and thrips, the vectors of tomato spotted wilt virus (TSWV). The objective of this research was to map the quantitative trait loci (QTLs) for resistance to leaf spots and TSWV in one recombinant inbred line (RIL) mapping population of “Tifrunner × GT-C20” for identification of linked markers for marker-assisted breeding. Here, we report the improved genetic linkage map with 418 marker loci with a marker density of 5.3 cM/loci and QTLs associated with multi-year (2010–2013) field phenotypes of foliar disease traits, including early leaf spot (ELS), late leaf spot (LLS), and TSWV. A total of 42 QTLs were identified with phenotypic variation explained (PVE) from 6.36 to 15.6%. There were nine QTLs for resistance to ELS, 22 QTLs for LLS, and 11 QTLs for TSWV, including six, five, and one major QTLs with PVE higher than 10% for resistance to each disease, respectively. Of the total 42 QTLs, 34 were mapped on the A sub-genome and eight mapped on the B sub-genome suggesting that the A sub-genome harbors more resistance genes than the B sub-genome. This genetic linkage map was also compared with two diploid peanut physical maps, and the overall co-linearity was 48.4% with an average co-linearity of 51.7% for the A sub-genome and 46.4% for the B sub-genome. The identified QTLs associated markers and potential candidate genes will be studied further for possible application in molecular breeding in peanut genetic improvement for disease resistance.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Tomato spotted wilt virus (TSWV), Early leaf spot (ELS), Late leaf spot (LLS), Quantitative trait locus (QTL), Peanuts, Groundnut
Subjects: Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 10 Mar 2017 11:18
Last Modified: 10 Mar 2017 11:18
URI: http://oar.icrisat.org/id/eprint/9904
Official URL: http://dx.doi.org/10.3389/fpls.2017.00025
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We thank Billy Wilson, Jake Fountain, Stephanie Lee, Lucero Gutierrez, Sara Beth Pelham, Victoria Weaver, and Jake Weawer for technical assistance in the field and the laboratory work. This research was partially supported by funds provided by the USDA Agricultural Research Service, the Georgia Peanut Commission, Peanut Foundation and National Peanut Board of USA and World Bank assisted Watershed Development Project-II (KWDP-II) by Government of Karnataka, India. This work has been also undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.
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