Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions

Upadhyaya, H D and Wang, Y H and Sastry, D V S S R and Dwivedi, S L and Prasad, P V V and Burrell, A M and Klein, R R and Morris, G P and Klein, P E (2016) Association mapping of germinability and seedling vigor in sorghum under controlled low-temperature conditions. Genome, 59 (2). pp. 137-145. ISSN 0831-2796

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Abstract

Sorghum is one of the world’s most important food, feed, and fiber crops as well as a potential feedstock for lignocellulosic bioenergy. Early-season planting extends sorghum’s growing season and increases yield in temperate regions. However, sorghum’s sensitivity to low soil temperatures adversely impacts seed germination. In this study, we evaluated the 242 accessions of the ICRISAT sorghum mini core collection for seed germination and seedling vigor at 12 °C as a measure of cold tolerance. Genome-wide association analysis was performed with approximately 162 177 single nucleotide polymorphism markers. Only one marker locus (Locus 7-2) was significantly associated with low-temperature germination and none with vigor. The linkage of Locus 7-2 to low-temperature germination was supported by four lines of evidence: strong association in three independent experiments, co-localization with previously mapped cold tolerance quantitative trait loci (QTL) in sorghum, a candidate gene that increases cold tolerance and germination rate when its wheat homolog is overexpressed in tobacco, and its syntenic region in rice co-localized with two cold tolerance QTL in rice. This locus may be useful in developing tools for molecular breeding of sorghums with improved low-temperature germinability.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Sorghum, association mapping, SNP, early-season cold tolerance
Subjects: Mandate crops > Sorghum
Depositing User: Mr Ramesh K
Date Deposited: 07 Jan 2017 09:23
Last Modified: 07 Jan 2017 09:23
URI: http://oar.icrisat.org/id/eprint/9829
Official URL: http://dx.doi.org/10.1139/gen-2015-0122
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: The study is supported in part by the International Crops Research Institute for the Semi-Arid Tropics and the University of Louisiana at Lafayette. This work has been undertaken as part of the CGIAR Research Program on Dryland Cereals. We thank the editor and the reviewers whose suggestions have greatly improved the manuscript.
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