Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression

Jadhav, A R and War, A R and Nikam, A N and Adhav, A S and Gupta, V S and Sharma, H C and Giri, A P and Tamhane, V A (2016) Capsicum annuum proteinase inhibitor ingestion negatively impacts the growth of sorghum pest Chilo partellus and promotes differential protease expression. Biochemistry and Biophysics Reports, 8. pp. 302-309. ISSN 2405-5808

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Abstract

Background Chilo partellus is an important insect pest infesting sorghum and maize. The larvae internalize in the stem, rendering difficulties in pest management. We investigated the effects of Capsicum annuum proteinase inhibitors (CanPIs) on C. partellus larvae by in-vitro and in-vivo experiments. Methods Recombinant CanPI-7 (with four-Inhibitory Repeat Domains, IRDs), -22 (two-IRDs) and insect proteinase activities were estimated by proteinase assays, dot blot assays and in gel activity assays. Feeding bioassays of lab reared C. partellus with CanPI-7 and -22 were performed. C. partellus proteinase gene expression was done by RT-PCR. In-silico structure prediction of proteinases and CanPI IRDs was carried out, their validation and molecular docking was done for estimating the interaction strength. Results Larval proteinases of C. partellus showed higher activity at alkaline pH and expressed few proteinase isoforms. Both CanPIs showed strong inhibition of C. partellus larval proteinases. Feeding bioassays of C. partellus with CanPIs revealed a dose dependent retardation of larval growth, reduction of pupal mass and fecundity, while larval and pupal periods increased significantly. Ingestion of CanPIs resulted in differential up-regulation of C. partellus proteinase isoforms, which were sensitive to CanPI-7 but were insensitive to CanPI-22. In-silico interaction studies indicated the strong interaction of IRD-9 (of CanPI-22) with Chilo proteinases tested. Conclusions Of the two PIs tested, CanPI-7 prevents induction of inhibitor insensitive proteinases in C. partellus so it can be explored for developing C. partellus tolerance in sorghum. General significance Ingestion of CanPIs, effectively retards C. partellus growth; while differentially regulating the proteinases.

Item Type: Article
Divisions: Research Program : Asia
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Chilo partellus; Plant-insect interaction; Proteinase inhibitors; Sorghum pests; Protease up-regulation; Capsicum annuum
Subjects: Mandate crops > Sorghum
Depositing User: Mr Ramesh K
Date Deposited: 26 Oct 2016 09:16
Last Modified: 24 Mar 2017 10:36
URI: http://oar.icrisat.org/id/eprint/9752
Official URL: http://dx.doi.org/10.1016/j.bbrep.2016.09.016
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: The authors thank Department of Science and Technology (DST), Science and Engineering Research Board (SERB), Government of India (Grant No. SB/YS/LS-132/2013) and Departmental Research and Development Programme (DRDP), Institute of Bioinformatics and Biotechnology (IBB), Savitribai Phule Pune University (SPPU), Pune, Maharashtra, India for funding this work. The contribution of Mr. Prashant Phulpagar and Mr. Tejas Kulkarni in initiating the characterization of insect enzymes is highly acknowledged.
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