Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers

Wang, H and Khera, P and Huang, B E and Yuan, M and Katam, R and Zhuang, W and Harris-Shultz, K and Moore, K M and Culbreath, A K and Zhang, X and Varshney, R K and Xie, L and Guo, B (2016) Analysis of genetic diversity and population structure of peanut cultivars and breeding lines from China, India and the US using simple sequence repeat markers. Journal of Integrative Plant Biology, 58 (5). pp. 452-465. ISSN 16729072

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Abstract

Cultivated peanut is grown worldwide as rich-source of oil and protein. A broad genetic base is needed for cultivar improvement. The objectives of this study were to develop highly informative simple sequence repeat (SSR) markers and to assess the genetic diversity and population structure of peanut cultivars and breeding lines from different breeding programs in China, India and the US. A total of 111 SSR markers were selected for this study, resulting in a total of 472 alleles. The mean values of gene diversity and polymorphic information content (PIC) were 0.480 and 0.429, respectively. Country-wise analysis revealed that alleles per locus in three countries were similar. The mean gene diversity in the US, China and India was 0.363, 0.489 and 0.47 with an average PIC of 0.323, 0.43 and 0.412, respectively. Genetic analysis using the STRUCTURE divided these peanut lines into two populations (P1, P2), which was consistent with the dendrogram based on genetic distance (G1, G2) and the clustering of principal component analysis. The groupings were related to peanut market types and the geographic origin with a few admixtures. The results could be used by breeding programs to assess the genetic diversity of breeding materials to broaden the genetic base and for molecular genetics studies.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Genetic diversity, Peanut cultivars, SSR markers, Molecular genetics studies
Subjects: Others > Plant Breeding
Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 27 Sep 2016 08:34
Last Modified: 17 May 2017 10:30
URI: http://oar.icrisat.org/id/eprint/9700
Official URL: http://dx.doi.org/10.1111/jipb.12380
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We thank Liming Yang, Jake Fountain and Billy Wilson for technical assistance in the greenhouse and the laboratory. This work is partially supported by the US Department of Agriculture Agricultural Research Service (USDA-ARS), the Georgia Agricultural Commodity Commission for Peanuts, Peanut Foundation and National Peanut Board. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes and USAID University Linkage Grant. ICRISAT is a member of CGIAR Consortium. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal opportunity provider and employer.
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