Studies on the proteins of the mutants of barley grain. 2. Fractionation and characterization of the alcohol-soluble proteins

Singh, U and Sastry, L V S (1977) Studies on the proteins of the mutants of barley grain. 2. Fractionation and characterization of the alcohol-soluble proteins. Journal of Agricultural and Food Chemistry, 25 (4). pp. 912-917. ISSN 0021-8561

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Abstract

Fractionation and characterization of the alcohol-soluble proteins of a barley variety, its mutants, and Hiproly, a high-protein and high-nutritive barley isolated from the world barley collection, indicate quantitative and qualitative changes in the mutants. The 35 % ethanol-soluble subfraction is higher in proportion in the mutants and Hiproly, and this could have resulted from the higher proportions of the polar amino acids in these proteins among which are also some limiting amino acids like lysine and threonine. The digestibility, as observed by the in vitro procedure, of this subfraction in all the varieties also is higher and, presumably as a result, the hordein fraction of these mutants also shows better digestibility. It appears that, in view of the presence of more polar amino acids which include lysine in this subfraction and better digestibility, its enrichment could be a nutritionally favorable index of better grain quality.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Barley grain
Subjects:	Others
Depositing User:	Mr Ramesh K
Date Deposited:	14 Sep 2016 06:42
Last Modified:	14 Sep 2016 06:42
URI:	http://oar.icrisat.org/id/eprint/9670
Official URL:	http://dx.doi.org/10.1021/jf60212a048
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	The authors wish to thank B. R. Murty, Project Director, Nuclear Research Laboratory, and M. S. Naik, Head of the Division of Biochemistry, for their encouragement; H. C. Bansal for supply of the seed material; and S. R. Chatterjee for amino acid analyses.One of us (US.) is grateful to the Indian Council of Agricultural Research for an award of a Senior Fellowship during the course of this investigation.
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