Comprehensive tissue-specific proteome analysis of drought stress responses in Pennisetum glaucum (L.) R. Br. (Pearl millet)

Ghatak, A and Chaturvedi, P and Nagler, M and Roustan, V and Lyon, D and Bachmann, G and Postl, W and Schröfl, A and Desai, N and Varshney, R K and Weckwerth, W (2016) Comprehensive tissue-specific proteome analysis of drought stress responses in Pennisetum glaucum (L.) R. Br. (Pearl millet). Journal of Proteomics, 143. pp. 122-135. ISSN 1876-7737

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Abstract

Pearl millet is the fifth most important cereal crop worldwide and cultivated especially by small holder farmers in arid and semi-arid regions because of its drought and salt tolerance. The molecular mechanisms of drought stress tolerance in Pennisetum remain elusive. We have used a shotgun proteomics approach to investigate protein signatures from different tissues under drought and control conditions. Drought stressed plants showed significant changes in stomatal conductance and increased root growth compared to the control plants. Root, leaf and seed tissues were harvested and 2281 proteins were identified and quantified in total. Leaf tissue showed the largest number of significant changes (120), followed by roots (25) and seeds (10). Increased levels of root proteins involved in cell wall-, lipid-, secondary- and signaling metabolism and the concomitantly observed increased root length point to an impaired shoot–root communication under drought stress. The harvest index (HI) showed a significant reduction under drought stress. Proteins with a high correlation to the HI were identified using sparse partial least square (sPLS) analysis. Considering the importance of Pearl millet as a stress tolerant food crop, this study provides a first reference data set for future investigations of the underlying molecular mechanisms.

Item Type: Article
Divisions: Research Program : Genetic Gains Program
CRPS: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Drought stress; Plant productivity; Global climate change; Arid and semi-arid regions; Multivariate statistics; Tissue-specific; Harvest index; Pearl millet
Subjects: Mandate crops > Millets
Mandate crops > Millets > Pearl Millet
Others > Drought
Depositing User: Mr Ramesh K
Date Deposited: 11 Aug 2016 11:26
Last Modified: 12 Aug 2016 07:52
URI: http://oar.icrisat.org/id/eprint/9605
Official URL: http://dx.doi.org/10.1016/j.jprot.2016.02.032
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We thank the European commission for the financial support of Palak Chaturvedi who is funded by the EU Marie-Curie International training network “Solanaceae pollen thermotolerance SPOT-ITN”, grant agreement number 289220. We thank the EU Marie-Curie International training network “CALIPSO”, grant number GA 2013-607-607, for financial support of Valentin Roustan. We thank the Austrian Science Fund (FWF) for financial support of Matthias Nagler by the Grant P 26342. We thank our gardeners and the glasshouse facility of the Department for Ecogenomics and Systems Biology for great support.
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