Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites

Galyuon, I K A and Madhusudhana, R and Borrell, A K and Hash, C T and Howarth, C J (2016) Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites. African Journal of Biotechnology, 15 (25). pp. 1363-1374. ISSN 1684-5315

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Abstract

The genetic variability of 28 sorghum genotypes of known senescence phenotype was investigated using 66 SSR markers well-distributed across the sorghum genome. The genotypes of a number of lines from breeding programmes for stay-green were also determined. This included lines selected phenotypically for stay-green and also RSG 03123, a marker-assisted backcross progeny of R16 (recurrent parent) and B35 (stay-green donor). A total of 419 alleles were detected with a mean of 6.2 per locus. The number of alleles ranged from one for Xtxp94 to 14 for Xtxp88. Chromosome SBI-10 had the highest mean number of alleles (8.33), while SBI-05 had the lowest (4.17). The PIC values obtained ranged from zero to 0.89 in Xtxp94 and Xtxp88, respectively, with a mean of 0.68. On a chromosome basis, mean PIC values were highest in SBI-10 (0.81) and lowest in SBI-05 (0.53). Most of the alleles from B35 in RSG 03123 were found on chromosomes SBI-01, SBI-02 and SBI-03, confirming the successful introgression of quantitative trait loci associated with stay-green from B35 into the senescent background R16. However, the alternative stay-green genetic sources were found to be distinct based on either all the SSRs employed or using only those associated with the stay-green trait in B35. Therefore, the physiological and biochemical basis of each stay-green source should be evaluated in order to enhance the understanding of the functioning of the trait in the various backgrounds. These genetic sources of stay-green could provide a valuable resource for improving this trait in sorghum breeding programmes.

Item Type: Article
Divisions: RP-Dryland Cereals
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Simple sequence repeats, Sorghum, Stay-green, Genetic diversity
Subjects: Mandate crops > Sorghum
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 27 Jul 2016 04:42
Last Modified: 21 Oct 2016 05:00
URI: http://oar.icrisat.org/id/eprint/9588
Official URL: http://dx.doi.org/10.5897/AJB2015.15074
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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