Galyuon, I K A and Madhusudhana, R and Borrell, A K and Hash, C T and Howarth, C J (2016) Genetic diversity of stay-green sorghums and their derivatives revealed by microsatellites. African Journal of Biotechnology, 15 (25). pp. 1363-1374. ISSN 1684-5315
|
PDF (It is an Open Access)
- Published Version
Download (532kB) | Preview |
Abstract
The genetic variability of 28 sorghum genotypes of known senescence phenotype was investigated using 66 SSR markers well-distributed across the sorghum genome. The genotypes of a number of lines from breeding programmes for stay-green were also determined. This included lines selected phenotypically for stay-green and also RSG 03123, a marker-assisted backcross progeny of R16 (recurrent parent) and B35 (stay-green donor). A total of 419 alleles were detected with a mean of 6.2 per locus. The number of alleles ranged from one for Xtxp94 to 14 for Xtxp88. Chromosome SBI-10 had the highest mean number of alleles (8.33), while SBI-05 had the lowest (4.17). The PIC values obtained ranged from zero to 0.89 in Xtxp94 and Xtxp88, respectively, with a mean of 0.68. On a chromosome basis, mean PIC values were highest in SBI-10 (0.81) and lowest in SBI-05 (0.53). Most of the alleles from B35 in RSG 03123 were found on chromosomes SBI-01, SBI-02 and SBI-03, confirming the successful introgression of quantitative trait loci associated with stay-green from B35 into the senescent background R16. However, the alternative stay-green genetic sources were found to be distinct based on either all the SSRs employed or using only those associated with the stay-green trait in B35. Therefore, the physiological and biochemical basis of each stay-green source should be evaluated in order to enhance the understanding of the functioning of the trait in the various backgrounds. These genetic sources of stay-green could provide a valuable resource for improving this trait in sorghum breeding programmes.
| Item Type: | Article |
|---|---|
| Divisions: | RP-Dryland Cereals |
| CRP: | CGIAR Research Program on Dryland Cereals |
| Uncontrolled Keywords: | Simple sequence repeats, Sorghum, Stay-green, Genetic diversity |
| Subjects: | Mandate crops > Sorghum Others > Genetics and Genomics |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 27 Jul 2016 04:42 |
| Last Modified: | 21 Feb 2018 08:30 |
| URI: | http://oar.icrisat.org/id/eprint/9588 |
| Official URL: | http://dx.doi.org/10.5897/AJB2015.15074 |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
| Links: |
Actions (login required)
 |
View Item |
Altmetric
Altmetric