QTL‐seq for rapid identification of candidate genes for 100‐seed weight and root/total plant dry weight ratio under rainfed conditions in chickpea

Singh, V K and Khan, A W and Jaganathan, D and Thudi, M and Roorkiwal, M and Takagi, H and Garg, V and Kumar, V and Chitikineni, A and Gaur, P M and Sutton, T and Terauchi, R and Varshney, R K (2016) QTL‐seq for rapid identification of candidate genes for 100‐seed weight and root/total plant dry weight ratio under rainfed conditions in chickpea. Plant Biotechnology Journal. pp. 2110-2119. ISSN 1467-7644

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Abstract

Terminal drought is a major constraint to chickpea productivity. Two component traits responsible for reduction in yield under drought stress include reduction in seeds size and root length/root density. QTL-seq approach, therefore, was used to identify candidate genomic regions for 100-seed weight (100SDW) and total dry root weight to total plant dry weight ratio (RTR) under rainfed conditions. Genomewide SNP profiling of extreme phenotypic bulks from the ICC 4958 × ICC 1882 population identified two significant genomic regions, one on CaLG01 (1.08 Mb) and another on CaLG04 (2.7 Mb) linkage groups for 100SDW. Similarly, one significant genomic region on CaLG04 (1.10 Mb) was identified for RTR. Comprehensive analysis revealed four and five putative candidate genes associated with 100SDW and RTR, respectively. Subsequently, two genes (Ca_04364 and Ca_04607) for 100SDW and one gene (Ca_04586) for RTR were validated using CAPS/dCAPS markers. Identified candidate genomic regions and genes may be useful for molecular breeding for chickpea improvement.

Item Type: Article
Divisions: Research Program : Genetic Gains
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Chickpea, Resequencing, Trait mapping, Seed weight, Root ratio, SNP-index, Genomic regions, Molecular breeding, Chickpea improvement
Subjects: Mandate crops > Chickpea
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 09 Jun 2016 08:50
Last Modified: 09 Feb 2017 05:58
URI: http://oar.icrisat.org/id/eprint/9544
Official URL: http://dx.doi.org/10.1111/pbi.12567
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: The work has been undertaken as a part of Australia–India strategic research fund (AISRF) project funded by Department of Science and Technology (DST) and Tropical Legumes (TLI & II) projects funded by Bill and Melinda Gates Foundation (BMGF). This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
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