Molecular evaluation of Ethiopian sweet sorghum germplasm and their contribution to regional breeding programs

Disasa, T and Feyissa, T and Admassu, B and Paliwal, R and Villiers, S D and Odeny, D A (2016) Molecular evaluation of Ethiopian sweet sorghum germplasm and their contribution to regional breeding programs. Australian Journal of Crop Science, 10 (04). pp. 520-527. ISSN 1835-2707

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Abstract

Sweet sorghum is an excellent feedstock for ethanol production and is also used for food and livestock feed. Germplasm collection and characterization in sweet sorghum is a crucial step towards breeding and development of superior genotypes for various end-uses. In the present study, 13 Simple Sequence Repeat (SSR) markers were used for genotyping 175 Ethiopian sweet sorghum genotypes alongside 27 improved accessions from eastern and southern Africa. All the tested markers detected 159 alleles and a high degree of polymorphism information content (PIC) averaging 0.69. A comparison between Ethiopian and improved accessions revealed higher allele numbers (124) in Ethiopian than improved accessions (92 alleles). More than half (65 out of 124) of the alleles observed in the Ethiopian accessions were rare (<5%) and 64 were private (only present within Ethiopian accessions) while in the improved accessions, 41% and 38% of the alleles detected were rare and private respectively. Both weighted Neighbor Joining-based clustering and hierarchical clustering grouped the 202 accessions into three major clusters based on geographical origin. Ethiopian accessions from the north (north Wello and south Tigray) not only clustered separately from accessions from the west central and eastern Ethiopia, but were also distinct from most of the improved genotypes. Our results reveal an unexploited highly diverse sweet sorghum genetic resource from Ethiopia that can be included in the regional breeding programs in order to efficiently optimize productivity.

Item Type: Article
Divisions: RP-Dryland Cereals
CRPS: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: AMOVA; Genetic diversity; Population structure; Sorghum bicolor; SSR markers; Molecular evaluation; Sweet sorghum; Germplasm ; Breeding programs
Subjects: Mandate crops > Sorghum
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 16 May 2016 09:48
Last Modified: 16 May 2016 09:48
URI: http://oar.icrisat.org/id/eprint/9524
Official URL: http://www.cropj.com/
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This research is part of a PhD work by the first author. The study was conducted by financial support provided by the Swedish International Development Agency (SIDA) through Bio-Innovate project “Delivering new sorghum and millets innovations for food security and improving livelihoods in Eastern Africa”- project No. 01/2010. The authors acknowledge additional financial support of the Ethiopian Institute of Agricultural Research. The authors are also grateful to Miss. Annis Saiyiorri, Mr. Vincent Njunge and Samuel Manthi for their technical assistance.
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