Identification of rust resistance in groundnut using a validated SSR marker

Yol, E and Upadhyaya, H D and Uzun, B (2016) Identification of rust resistance in groundnut using a validated SSR marker. Euphytica, 210. pp. 405-410. ISSN 0014-2336

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Abstract

Groundnut (Arachis hypogaea L.) is an important crop cultivated in over 100 countries in the world. The rust disease of groundnut, caused by Puccinia arachidis Speg., can cause significant yield losses in tropical and subtropical areas. The disease affects not only seed yield but also fodder yield and quality. There are chemicals available to control rust; however, the development of resistant varieties is the most reasonable way to improve yield and quality, and to reduce the adverse effects of chemicals on the ecosystem. Characterization of germplasm diversity to identify resistant sources using traditional methods is a lengthy process and requires laborious field testing. Molecular marker-aided selection offers an alternative breeding method that is relatively easy, precise, and not affected by environmental fluctuation. In the present study, a validated SSR marker, GM1954, linked to the rust disease resistance gene was used for 256 groundnut genotypes to select rust resistance. This study reports the successful application of marker-assisted selection for further rust-resistant breeding programs in groundnut. Molecular analyses revealed that the banding pattern related to disease resistance was observed at high frequency in the variety hypogaea among the nine identified resistant genotypes in the collection. Approximately 3 % of the collection was selected for further field, greenhouse, and hybridization experiments.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Characterization, Fungal disease, Marker-aided selection, Molecular markers, Puccinia arachidis Speg
Subjects: Mandate crops > Groundnut
Depositing User: Mr Ramesh K
Date Deposited: 10 May 2016 09:22
Last Modified: 21 Oct 2016 09:27
URI: http://oar.icrisat.org/id/eprint/9504
Official URL: http://dx.doi.org/10.1007/s10681-016-1705-3
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This study was supported by the Ministry of Science, Industry and Technology of Turkey and the Scientific Research Projects Coordination Unit of Akdeniz University (grants SANTEZ- 01527-STZ-2012-2 and FDK- 2014-140, respectively). We are grateful to International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Gene bank, Hyderabad, India for supplying genetic material several times. We appreciate Allan Booth of The James Hutton Institute, Dundee, UK for his critical editing of the manuscript.
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