Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.)

Sinha, P and Pazhamala, L T and Singh, V K and Saxena, R K and Krishnamurthy, L and Azam, S and Khan, A W and Varshney, R K (2016) Identification and Validation of Selected Universal Stress Protein Domain Containing Drought-Responsive Genes in Pigeonpea (Cajanus cajan L.). Frontiers in Plant Science, 06 (1065). 01-10. ISSN 1664-462X

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Abstract

Pigeonpea is a resilient crop, which is relatively more drought tolerant than many other legume crops. To understand the molecular mechanisms of this unique feature of pigeonpea, 51 genes were selected using the Hidden Markov Models (HMM) those codes for proteins having close similarity to universal stress protein domain. Validation of these genes was conducted on three pigeonpea genotypes (ICPL 151, ICPL 8755, and ICPL 227) having different levels of drought tolerance. Gene expression analysis using qRT-PCR revealed 6, 8, and 18 genes to be ≥2-fold differentially expressed in ICPL 151, ICPL 8755, and ICPL 227, respectively. A total of 10 differentially expressed genes showed ≥2-fold up-regulation in the more drought tolerant genotype, which encoded four different classes of proteins. These include plant U-box protein (four genes), universal stress protein A-like protein (four genes), cation/H(+) antiporter protein (one gene) and an uncharacterized protein (one gene). Genes C.cajan_29830 and C.cajan_33874 belonging to uspA, were found significantly expressed in all the three genotypes with ≥2-fold expression variations. Expression profiling of these two genes on the four other legume crops revealed their specific role in pigeonpea. Therefore, these genes seem to be promising candidates for conferring drought tolerance specifically to pigeonpea.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Pigeonpea, Genome sequence, Gene expression, Drought tolerance, Protein Domain
Subjects: Mandate crops > Pigeonpea
Depositing User: Mr Ramesh K
Date Deposited: 29 Apr 2016 06:40
Last Modified: 21 Oct 2016 09:01
URI: http://oar.icrisat.org/id/eprint/9461
Official URL: http://dx.doi.org/10.3389/fpls.2015.01065
Projects: UNSPECIFIED
Funders: USAID
Acknowledgement: The authors thank United States Agency for International Development (USAID) for financial support for the research work. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
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