Transcriptome-wide sequencing provides insights into geocarpy in peanut (Arachis hypogaea L.)

Chen, X and Yang, Y and Li, H and Li, H and Hong, Y and Pan, L and Chen, N and Zhu, F and Chi, X and Zhu, W and Chen, M and Liu, H and Yang, Z and Zhang, E and Wang, T and Zhong, N and Wang, M and Liu, H and Wen, S and Li, X and Zhou, G and Li, S and Wu, H and Varshney, R K and Liang, X and Yu, S (2016) Transcriptome-wide sequencing provides insights into geocarpy in peanut (Arachis hypogaea L.). Plant Biotechnology Journal, 14 (05). pp. 1215-1224. ISSN 1467-7652

[img]
Preview
PDF (It is an Open Access article) - Published Version
Download (1MB) | Preview

Abstract

A characteristic feature of peanut is the subterranean fructification, geocarpy, in which the gynophore (‘peg’), a specialized organ that transitions from upward growth habit to downward outgrowth upon fertilization, drives the developing pod into the soil for subsequent development underground. As a step towards understanding this phenomenon, we explore the developmental dynamics of the peanut pod transcriptome at 11 successive stages. We identified 110 217 transcripts across developmental stages and quantified their abundance along a pod developmental gradient in pod wall. We found that the majority of transcripts were differentially expressed along the developmental gradient as well as identified temporal programs of gene expression, including hundreds of transcription factors. Thought to be an adaptation to particularly harsh subterranean environments, both up- and down-regulated gene sets in pod wall were enriched for response to a broad array of stimuli, like gravity, light and subterranean environmental factors. We also identified hundreds of transcripts associated with gravitropism and photomorphogenesis, which may be involved in the geocarpy. Collectively, this study forms a transcriptional baseline for geocarpy in peanut as well as provides a considerable body of evidence that transcriptional regulation in peanut aerial and subterranean fruits is complex.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Peanut (Archis hypogaea L.), RNA-Seq, Geocarpy, Groundnut, Fruit development
Subjects: Mandate crops > Groundnut
Depositing User: Mr Ramesh K
Date Deposited: 18 Apr 2016 10:35
Last Modified: 21 Oct 2016 09:26
URI: http://oar.icrisat.org/id/eprint/9438
Official URL: http://dx.doi.org/10.1111/pbi.12487
Projects: UNSPECIFIED
Funders: Science and Technology Planning Project of Guangdong Province, Pearl River Science and Technology Nova of Guangzhou, National Natural Science Foundation of China, Natural Science Foundation of Guangdong Province, Modern Agro-industry Technology Research S
Acknowledgement: This research was funded by grants from Science and Technology Planning Project of Guangdong Province (No. 2012B050700007, 2011B010500019, 2013B050800021), Pearl River Science and Technology Nova of Guangzhou (No. 2011J2200035, 2013J2200088), National Natural Science Foundation of China (No. 31200155, 31501246, 31271767) and Natural Science Foundation of Guangdong Province (S2013020012647) and supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-14). The founders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. We declare no conflict of interests.
Links:

Actions (login required)

View Item View Item