Analysis of genetic variation in sorghum (Sorghum bicolor (L.) Moench) genotypes with various agronomical traits using SPAR methods

Satish, L and Shilpha, J and Pandian, S and Rency, A S and Rathinapriya, P and Ceasar, S A and Largia, M J V and Ashok Kumar, A and Ramesh, M (2016) Analysis of genetic variation in sorghum (Sorghum bicolor (L.) Moench) genotypes with various agronomical traits using SPAR methods. Gene, 576 (01 (3)). pp. 581-585. ISSN 1879-0038

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Genetic variation among 45 genotypes of sorghum (Sorghum bicolor L.) representing seven subpopulations was assessed using three single primer amplification reaction (SPAR) methods viz., inter-simple sequence repeat (ISSR), random amplified polymorphic DNA (RAPD) and directed amplification of minisatellite-region DNA (DAMD). Totally 15 ISSR, 8 RAPD and 7 DAMD primers generated 263 amplification products, accounting for 84.6% polymorphism across all the genotypes. The Mantel's test of correlation revealed the best correlation between ISSR and cumulative data with a correlation coefficient (r) of 0.84. Assessment of population diversity indicated that the maximum intra population genetic diversity was recorded among high FeZn lines (HFL) having maximum values of Nei's genetic diversity (h) (0.244), Shannon information index (I) (0.368) and the percentage of polymorphic loci (Pp) (72.65%) while the corresponding lowest values of 0.074, 0.109 and 17.95% respectively were observed among the members of MDT subpopulation. The mean coefficient of gene differentiation (GST) and the gene flow (Nm) between populations were observed to be 0.396 and 0.7680 respectively. The analysis of molecular variance (AMOVA) suggested that maximum genetic variation exists within populations (95%) than among populations (5%). Thus the information obtained from this study could be utilized in sorghum breeding programmes for the development of varieties with improved nutrition and agronomic values in future.

Item Type: Article
Divisions: RP-Dryland Cereals
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: DAMD; Genetic variation; ISSR; RAPD; Sorghum; SPAR methods; Sorghum breeding
Subjects: Mandate crops > Sorghum
Depositing User: Mr Ramesh K
Date Deposited: 13 Apr 2016 10:27
Last Modified: 21 Oct 2016 05:02
Official URL:
Funders: Department of Biotechnology, Government of India; Grant No.BT/BI/25/001/2006
Acknowledgement: The author L. Satish sincerely thanks the University Grants Commission, New Delhi, India for financial support in the form of UGC BSR SRF (UGC order no. F.4-1/2006 (BSR)/7-326/2011(BSR), dated 25.02.2013). We thank Sorghum Breeding, International CropsResearch Institute for the Semi-AridTropics(ICRISAT), Hyderabad, India, and the Department of Small Millets, Millet Research Station, Tamil Nadu Agricultural University (TNAU), Coimbatore, India for providing the seed materials used in this study. Also the authors gratefully acknowledge the computational and bioinformatics facility provided by the Alagappa University Bioinformatics Infrastructure Facility (funded by Department of Biotechnology, Government of India; Grant No.BT/BI/25/001/2006).
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