Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]

Gnanesh, B N and Bohra, A and Sharma, M and Byregowda, M and Pandey, S and Wesley, V and Saxena, R K and Saxena, K B and Kavi Kishor, P B and Varshney, R K (2011) Genetic mapping and quantitative trait locus analysis of resistance to sterility mosaic disease in pigeonpea [Cajanus cajan (L.) Millsp.]. Field Crops Research, 123. pp. 53-61.

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Abstract

Sterility mosaic disease (SMD), considered as the “green plague of pigeonpea” and caused by pigeonpea sterility mosaic virus (PPSMV) is one of the major biotic factors, which leads to heavy yield losses and hence poses a big challenge for pigeonpea production in the Indian subcontinent. Variability in the sterility mosaic pathogen revealed the occurrence of five different isolates in India. Among them, three distinct SMD isolates have been characterized, viz., Patancheru, Bangalore and Coimbatore. Molecular tools offer a viable option to tackle these biotic stresses via identification of the genomic regions associated with the trait such as SMD resistance. With an aim of identifying the gene(s)/QTLs linked with SMD resistance, two F2 populations, i.e. ICP 8863×ICPL 20097 (segregating for Patancheru SMD isolate) and TTB 7×ICP 7035 (segregating for both Patancheru and Bangalore SMD isolates) were developed and F2:3 families were phenotyped for resistance to respective isolate(s) of SMD. After screening over 3000 SSR markers on parental genotypes of each mapping population, intra-specific genetic maps comprising of 11 linkage groups and 120 and 78 SSR loci were developed for ICP 8863×ICPL 20097 and TTB 7×ICP 7035 populations, respectively. Composite interval mapping (CIM) based QTL analysis by using genetic mapping and phenotyping data provided four QTLs for Patancheru SMD isolate and two QTLs for Bangalore SMD isolate. Identification of different QTLs for resistance to Patancheru and Bangalore SMD isolates is an indication of involvement of different genes conferring the resistance to these two SMD isolates. One QTL namely qSMD4 identified within an interval of 2.8cM on LG 7 explaining 24.72% of phenotypic variance, once it is validated in other genetic background, seems to be a promising QTL for use in marker assisted selection. In summary, this is the first study on development of intra-specific genetic maps and identification of QTLs for SMD resistance in pigeonpea

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Pigeonpea; Molecular markers; Simple sequence repeats (SSRs);Linkage map; QTL
Agro Tags:	<b>Agrotags</b> - genetics | isolation | genetic maps | planting | social groups | diseases | crops | phenotypes | genomes | dna <br><b>Fishtags</b> - NOT-AVAILABLE<br><b>Geopoliticaltags</b> - india | usa | netherlands | mexico
Subjects:	Mandate crops > Pigeonpea
Depositing User:	Siva Shankar
Date Deposited:	21 Jun 2011 14:45
Last Modified:	21 Jun 2011 14:45
URI:	http://oar.icrisat.org/id/eprint/94
Official URL:	http://dx.doi.org/10.1016/j.fcr.2011.04.011
Projects:	UNSPECIFIED
Funders:	Indian Council of Agricultural Research, Government of India - Department of Biotechnology, Generation Challenge Programme
Acknowledgement:	Authors are thankful to the Indo-US Agricultural Knowledge Initiative of Indian Council of Agricultural Research (ICAR) and Department of Biotechnology (DBT) of the Government of India and CGIAR Generation Challenge Programme for financial support to undertake this study. Thanks are also due to Dr Abhishek Rathore, ICRISAT; Dr T. Nepolean, Indian Agricultural Research Institute (IARI), New Delhi; Dr K. N. Ganapathy, Directorate of Sorghum Research (DSR), Hyderabad and Dr Y. P. Khedikar, University of Saskatchewan, Canada, for their valuable suggestions.
Links:	Author

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