Jakkeral, S A and Nadaf, H L and Gowda, M V C and Bhat, R S and Motagi, B N and Mukri, G and Gangashetty, P I and Talawar, A and Archana, B (2014) Genetic variability and marker detection for rust resistance in recombinant inbred lines and backcross inbred lines of groundnut (Arachis hypogaea l.). In: Advances in Arachis Through Genomlcs & Biotechnology (AAGB 2014), 7th International Conference of the Peanut Research Community, November 11-14, 2014, Savannah Marriott Riverfront, Savannah, Georgia, USA.
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Abstract
A F6 mapping population and backcross populations (BC1F4 and BC2F3 BC3F2) were developed from the cross between the susceptible parent GPBD-5 and resistant parents ICGV 86699 and ICGV 99005 to dissect the genetic variation and SSR markers linked to the rust resistance in groundnut. Genetic variability revealed that there were highly significant differences among recombinants for rust reaction. Less differences were observed between PCV and GCV for rust reaction in both crosses, which indicated a greater role of genetic components. High values of heritability (>80%) genetic advance and-genetic advance as percent mean was observed for rust reaction in F6 and backcross populations. Bulk segregant analysis in the segregating populations of both crosses (GPBD-5 x ICGV 86699 and GPBD-5 x ICGV 99005) indicated Tc4g10 marker was putatively linked to the rust resistant gene. The association of the putative marker identified based on DNA pooling from the selected segregants was established by single marker analysis (SMA). In the F6 population of both crosses GPBD-5 x ICGV 86699 and GPBD-5 x ICGV 99005, the Tc4glO marker accounted for 72.40% and 50.60% total variation, respectively. Tc4g10 marker accounted for 67.10%,38.40% and 61.30% total variation in the cross GPBD-5 x ICGV 86699, and the same marker accounted for 73.8%, 54.7% and 84.4% total variation in the cross GPBD-5 x ICGV 99005 in BC1F4, BC2F3 and BC3F2, respectively. This marker can be used in marker assisted selection for rust resistance in groundnut improvement programs.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | RP-Grain Legumes |
| CRP: | CGIAR Research Program on Grain Legumes |
| Uncontrolled Keywords: | Genetic variability, Rust resistance, Groundnut |
| Subjects: | Mandate crops > Groundnut Others > Genetics and Genomics |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 16 Mar 2016 10:50 |
| Last Modified: | 07 Sep 2017 06:12 |
| URI: | http://oar.icrisat.org/id/eprint/9390 |
| Acknowledgement: | UNSPECIFIED |
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