A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut (Arachis hypogaea L.)

    Khedikar, Y P and Gowda, M V C and Sarvamangala, C and Patgar, K V and Upadhyaya, H D and Varshney, R K (2010) A QTL study on late leaf spot and rust revealed one major QTL for molecular breeding for rust resistance in groundnut (Arachis hypogaea L.). Theoretical and Applied Genetics, 121 (05). pp. 971-984. ISSN 1432-2242

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    Abstract

    Late leaf spot (LLS) and rust are two major foliar diseases of groundnut (Arachis hypogaea L.) that often occur together leading to 50–70% yield loss in the crop. A total of 268 recombinant inbred lines of a mapping population TAG 24 × GPBD 4 segregating for LLS and rust were used to undertake quantitative trait locus (QTL) analysis. Phenotyping of the population was carried out under artificial disease epiphytotics. Positive correlations between different stages, high to very high heritability and independent nature of inheritance between both the diseases were observed. Parental genotypes were screened with 1,089 simple sequence repeat (SSR) markers, of which 67 (6.15%) were found polymorphic. Segregation data obtained for these markers facilitated development of partial linkage map (14 linkage groups) with 56 SSR loci. Composite interval mapping (CIM) undertaken on genotyping and phenotyping data yielded 11 QTLs for LLS (explaining 1.70–6.50% phenotypic variation) in three environments and 12 QTLs for rust (explaining 1.70–55.20% phenotypic variation). Interestingly a major QTL associated with rust (QTLrust01), contributing 6.90–55.20% variation, was identified by both CIM and single marker analysis (SMA). A candidate SSR marker (IPAHM 103) linked with this QTL was validated using a wide range of resistant/susceptible breeding lines as well as progeny lines of another mapping population (TG 26 × GPBD 4). Therefore, this marker should be useful for introgressing the major QTL for rust in desired lines/varieties of groundnut through marker-assisted backcrossing.

    Item Type: Article
    Divisions: RP-Grain Legumes
    CRP: CGIAR Research Program on Grain Legumes
    Uncontrolled Keywords: Biotechnology, Biochemistry, Plant Biochemistry, Agriculture, Plant Genetics & Genomics, Plant Breeding, Groundnut
    Subjects: Mandate crops > Groundnut
    Others > Genetics and Genomics
    Depositing User: Mr Ramesh K
    Date Deposited: 03 Mar 2016 08:55
    Last Modified: 03 Mar 2016 08:55
    URI: http://oar.icrisat.org/id/eprint/9361
    Official URL: http://dx.doi.org/10.1007/s00122-010-1366-x
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: Authors are thankful to the National Fund of the Basic and Strategic Research in Agriculture (NFBSRA) of the Indian Council of Agriculture (ICAR-RKV, MVCG), Department of Biotechnology (DBT) of the Government of India (MVCG, RKV) and the Tropical Legume I of Generation Challenge Programme (RKV) for Wnancial support to undertake this study. Thanks are also due to Dr T. Nepolean of the Indian Agricultural Research Institute (IARI), New Delhi, Dr P. L. Kulwal of Mahatma Phule Krishi Vidyapeeth (MPKV), Rahuri and the two anonymous reviewers for their valuable suggestions.
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