Garg, R and Shankar, R and Thakkar, B and Kudapa, H and Krishnamurthy, L and Mantri, N and Varshney, R K and Bhatia, S and Jain, M (2016) Transcriptome analyses reveal genotype- and developmental stage-specific molecular responses to drought and salinity stresses in chickpea. Scientific Reports, 6 (19228). 01-15. ISSN 2045-2322
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Abstract
Drought and salinity are the major factors that limit chickpea production worldwide. We performed whole transcriptome analyses of chickpea genotypes to investigate the molecular basis of drought and salinity stress response/adaptation. Phenotypic analyses confirmed the contrasting responses of the chickpea genotypes to drought or salinity stress. RNA-seq of the roots of drought and salinity related genotypes was carried out under control and stress conditions at vegetative and/or reproductive stages. Comparative analysis of the transcriptomes revealed divergent gene expression in the chickpea genotypes at different developmental stages. We identified a total of 4954 and 5545 genes exclusively regulated in drought-tolerant and salinity-tolerant genotypes, respectively. A significant fraction (~47%) of the transcription factor encoding genes showed differential expression under stress. The key enzymes involved in metabolic pathways, such as carbohydrate metabolism, photosynthesis, lipid metabolism, generation of precursor metabolites/energy, protein modification, redox homeostasis and cell wall component biogenesis, were affected by drought and/or salinity stresses. Interestingly, transcript isoforms showed expression specificity across the chickpea genotypes and/or developmental stages as illustrated by the AP2-EREBP family members. Our findings provide insights into the transcriptome dynamics and components of regulatory network associated with drought and salinity stress responses in chickpea.
| Item Type: | Article |
|---|---|
| Divisions: | RP-Grain Legumes |
| CRP: | CGIAR Research Program on Grain Legumes |
| Uncontrolled Keywords: | Chickpea, Legume, Molecular Responses, Drought, Chickpea Production, Salinity Stresses, Genotypes, Genomics |
| Subjects: | Mandate crops > Chickpea Others > Genetics and Genomics |
| Depositing User: | Mr Ramesh K |
| Date Deposited: | 22 Jan 2016 05:45 |
| Last Modified: | 21 Oct 2016 09:30 |
| URI: | http://oar.icrisat.org/id/eprint/9276 |
| Official URL: | http://dx.doi.org/10.1038/srep19228 |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | This work was supported by the Ministry of Science and Technology, Government of India, New Delhi, under the Australia-India Strategic Research Fund (DST/INT/AUS/GCP-1/11 from 2012 to 2015) scheme. RG acknowledges INSPIRE Faculty Award from the Department of Science and Technology, Government of India, New Delhi. RS acknowledges the award of research fellowship from the Department of Biotechnology, Government of India. |
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