Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding.

Ellur, R K and Khanna, A and Yadav, A and Pathania, S and Rajashekara, H and Singh, V K and Gopala Krishnan, S and Bhowmick, P K and Nagarajan, M and Vinod, K K and Prakash, G and Mondal, K K and Singh, N K and Vinod Prabhu, K and Singh, A K (2016) Improvement of Basmati rice varieties for resistance to blast and bacterial blight diseases using marker assisted backcross breeding. Plant Science, 242. pp. 330-341. ISSN 0168-9452

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Abstract

Marker assisted backcross breeding was employed to incorporate the blast resistance genes, Pi2 and Pi54 and bacterial blight (BB) resistance genes xa13 and Xa21 into the genetic background of Pusa Basmati 1121 (PB1121) and Pusa Basmati 6. Foreground selection for target gene(s) was followed by arduous phenotypic and background selection which fast-tracked the recovery of recurrent parent genome (RPG) to an extent of 95.8% in one of the near-isogenic lines (NILs) namely, Pusa 1728-23-33-31-56, which also showed high degree of resemblance to recurrent parent, PB6 in phenotype. The phenotypic selection prior to background selection provided an additional opportunity for identifying the novel recombinants viz., Pusa 1884-9-12-14 and Pusa 1884-3-9-175, superior to parental lines in terms of early maturity, higher yield and improved quality parameters. There was no significant difference between the RPG recovery estimated based on SSR or SNP markers, however, the panel of SNPs markers was considered as the better choice for background selection as it provided better genome coverage and included SNPs in the genic regions. Multi-location evaluation of NILs depicted their stable and high mean performance in comparison to the respective recurrent parents. The Pi2+Pi54 carrying NILs were effective in combating a pan-India panel of Magnaporthe oryzae isolates with high level of field resistance in northern, eastern and southern parts of India. Alongside, the PB1121-NILs and PB6-NILs carrying BB resistance genes xa13+Xa21 were resistant against Xanthomonas oryzae pv. oryzae races of north-western, southern and eastern parts of the country. Three of NILs developed in this study, have been promoted to final stage of testing during the ​Kharif 2015 in the Indian National Basmati Trial.

Item Type: Article
Divisions: RP-Grain Legumes
CRPS: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Bacterial blight; Basmati rice; Blast; Gene pyramiding; Marker assisted backcross breeding
Subjects: Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 29 Dec 2015 08:15
Last Modified: 29 Dec 2015 08:19
URI: http://oar.icrisat.org/id/eprint/9204
Official URL: http://dx.doi.org/10.1016/j.plantsci.2015.08.020
Projects: UNSPECIFIED
Funders: Accelerated Crop Improve-ment Program, Department of Biotechnology, India
Acknowledgement: The study is part of the Ph.D. research of the first author. We acknowledge AllIndia Co-ordinated Rice improvement program, Indian Institute ofRice Research, Hyderabad for facilitating the evaluation of NILsunder national Basmati trial.
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