Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system

Kaushal, M and Kaushal, R (2015) Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system. Indian Journal of Biotechnology, 14 (2). pp. 221-227. ISSN 0975-0967

[img] PDF - Published Version
Restricted to ICRISAT users only

Download (361kB) | Request a copy

Abstract

Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 & MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 ηmole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29% over recommended doses of NPK with a saving of 25% N and 25% P fertilizers.

Item Type: Article
Divisions: RP-Resilient Dryland Systems
CRP: CGIAR Research Program on Dryland Systems
Uncontrolled Keywords: Acetylene reduction assay (ARA), Bacillus spp., Biocontrol, Cauliflower, INM, PGPR, 16S rRNA
Subjects: Others > Food and Nutrition
Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Mr B K Murthy
Date Deposited: 09 Nov 2015 09:15
Last Modified: 09 Nov 2015 09:15
URI: http://oar.icrisat.org/id/eprint/9122
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
Links:
View Statistics

Actions (login required)

View Item View Item