Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system

Kaushal, M and Kaushal, R (2015) Acetylene reductase activity and molecular characterization of plant growth promoting rhizobacteria to know efficacy in integrated nutrient management system. Indian Journal of Biotechnology, 14 (2). pp. 221-227. ISSN 0975-0967

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Abstract

Isolates from rhizosphere were screened and characterized for their plant growth promotion and biocontrol properties as per standard methods. Five isolates (MK2, MK4, MK5, MK7 & MK9) showed the maximum plant growth promoting attributes like P-solubilization, N-fixation etc. Acetylene reduction assay (ARA) method was used to study N2 fixing ability. The bacterial isolate MK5 fixed the maximum nitrogen, i.e., 437.26 ηmole C2H4 h-1 mg-1 protein (109 mg of N2 fixed/ha/d). The isolates MK5 and MK7 showed the maximum homology with Bacillus pumilus and B. subtilis, respectively by 16S rRNA analysis. The bacterial isolate MK5 in conjunction with 75% level of recommended dose of N and P fertilizers gave increase yield of cauliflower crop by 27.29% over recommended doses of NPK with a saving of 25% N and 25% P fertilizers.

Item Type:	Article
Divisions:	RP-Resilient Dryland Systems
CRP:	CGIAR Research Program on Dryland Systems
Uncontrolled Keywords:	Acetylene reduction assay (ARA), Bacillus spp., Biocontrol, Cauliflower, INM, PGPR, 16S rRNA
Subjects:	Others > Food and Nutrition Others > Agriculture-Farming, Production, Technology, Economics
Depositing User:	Mr B K Murthy
Date Deposited:	09 Nov 2015 09:15
Last Modified:	09 Nov 2015 09:15
URI:	http://oar.icrisat.org/id/eprint/9122
Official URL:
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

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