Construction of a genetic map for pearl millet, Pennisetum glaucum (L.) R. Br., using a genotyping-by-sequencing (GBS) approach

Moumouni, K H and Kountche, B A and Jean, M and Hash, C T and Vigouroux, Y and Haussmann, B I G and Belzile, F (2015) Construction of a genetic map for pearl millet, Pennisetum glaucum (L.) R. Br., using a genotyping-by-sequencing (GBS) approach. Molecular Breeding, 35 (5). pp. 1-10. ISSN 1380-3743

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Abstract

Pearl millet is the main component of traditional farming systems and a staple grain in the diet of sub-Saharan Africa and India. To facilitate breeding work in this crop, a genetic map consisting of single nucleotide polymorphism (SNP) markers was constructed using an F2 population of 93 progenies, from a wild × cultivated pearl millet cross. We used a modified genotyping-by-sequencing (GBS) protocol involving two restriction enzymes (PstI–MspI) and PCR amplification with primers including three selective bases to generate 3,321 SNPs. Of these, 2,809 high-quality SNPs exhibited a minor allele frequency ≥0.3. In total, 314 non-redundant haplotypes and 85 F2 individuals were used to construct a genetic map spanning a total distance of 640 cM. These SNPs were evenly distributed over seven linkage groups ranging considerably in size (62–123 cM). The average density for this map was 0.51 SNP/cM, and the average interval between SNP markers was 2.1 (±0.6) cM. Finally, to establish bridges between the linkage groups of this and previous maps, 19 SSR markers were examined for polymorphism between the parents of this population. We could only tentatively suggest a correspondence between four of our linkage groups and those of previous maps. Overall, GBS enabled us to quickly produce a genetic map with a density and uniformity of markers greater than previously published maps. The availability of such a map will be useful for the identification of genomic regions associated with Striga resistance and other important agronomic traits.

Item Type: Article
Divisions: RP-Dryland Cereals
CRPS: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Pearl millet, Genetic map, SNP, Genotyping-by-sequencing
Subjects: Mandate crops > Millets
Others > Genetics and Genomics
Depositing User: Mr B K Murthy
Date Deposited: 04 Nov 2015 03:45
Last Modified: 04 Nov 2015 03:45
URI: http://oar.icrisat.org/id/eprint/9112
Official URL: http://dx.doi.org/10.1007/s11032-015-0212-x
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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