Molecular cloning and characterization of salt inducible dehydrin gene from the C4 plant Pennisetum glaucum

Singh, J and Reddy, P S and Reddy, C S and Reddy, M K (2015) Molecular cloning and characterization of salt inducible dehydrin gene from the C4 plant Pennisetum glaucum. Plant Gene. 01-27. ISSN 2352-4073

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Abstract

Dehydrins (DHNs) or group 2 LEA (Late Embryogenesis Abundant) proteins play a protective role in plants under different abiotic stress conditions like drought, salinity, cold and heat stress. DHNs are expressed in late embryogenesis and accumulate in vegetative tissues in response to desiccation stress in all photosynthetic organisms. Here we report the cloning and characterization of a PgDHN gene from the C4 plant Pennisetum glaucum. The PgDHN codes for a polypeptide of 133 amino acids with an estimated molecular weight of 13.87 KDa and isoelectric point of 6.81. The protein sequence analysis of PgDHN classified it into the YnSKn subgroup of dehydrins. Phylogenetic analysis revealed that PgDHN is evolutionarily related to a Setaria italica DHN. In silico sequence analysis of the PgDHN promoter identified a distinct set of cis-elements and transcription factor binding sites. PgDHN mRNA accumulated in leaves of P. glaucum upon treatment with NaCl stress. Recombinant PgDHN transformed E. coli cells showed improved tolerance and exhibited better growth rate under high salt concentration (750 mM) and heat stress in comparison to their respective controls. Heterologus expression of PgDHN in transgenic yeast showed increased tolerance to multiple abiotic stresses. This study provides evidence that suggests the role of PgDHN in stress adaptation and stress tolerance in pearl millet.

Item Type: Article
Divisions: RP-Dryland Cereals
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Dehydrin; Pearl millet; Genome walking; Salt stress; Cis-elements; qRT-PCR
Subjects: Mandate crops > Millets > Pearl Millet
Depositing User: Mr Ramesh K
Date Deposited: 30 Sep 2015 06:19
Last Modified: 21 Oct 2016 04:52
URI: http://oar.icrisat.org/id/eprint/9028
Official URL: http://dx.doi.org/10.1016/j.plgene.2015.08.002
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: The Department of Biotechnology (DBT) Govt. of India, New Delhi, supported this study. Dr. Palakolanu SudhakarReddy acknowledge the award of INSPIRE Faculty (Grant No. IFA-11-LSPA-06) from DST, Govt. of India, New Delhi.
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