Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes

Janila, P and Pandey, M K and Shasidhar, Y and Variath, M T and Sriswathi, M and Khera, P and Manohar, S S and Nagesh, P and Vishwakarma, M K and Mishra, G P and Radhakrishnan, T and Manivannan, N and Dobariya, K L and Vasanthi, R P and Varshney, R K (2016) Molecular breeding for introgression of fatty acid desaturase mutant alleles (ahFAD2A and ahFAD2B) enhances oil quality in high and low oil containing peanut genotypes. Plant Science. pp. 203-213. ISSN 0168-9452

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Abstract

High oleate peanuts have two marketable benefits, health benefits to consumers and extended shelf life of peanut products. Two mutant alleles present on linkage group a09 ( ahFAD2A) and b09 ( ahFAD2B) control composition of three major fatty acids, oleic, linoleic and palmitic acids which together determine peanut oil quality. In conventional breeding, selection for fatty acid composition is delayed to advanced generations. However by using DNA markers, breeders can reject large number of plants in early generations and therefore can optimize time and resources. Here, two approaches of molecular breeding namely marker-assisted backcrossing (MABC) and marker-assisted selection (MAS) were employed to transfer two FAD2mutant alleles from SunOleic 95R into the genetic background of ICGV 6110, ICGV 6142 and ICGV 6420. In summary, 82 MABC and 387 MAS derived introgression lines (ILs) were developed using DNA markers with elevated oleic acid varying from 62 to 83%. Oleic acid increased by 0.5-1.1 folds, with concomitant reduction of linoleic acid by 0.4-1.0 folds and palmitic acid by 0.1-0.6 folds among ILs compared to recurrent parents. Finally, high oleate ILs, 27 with high oil (53.0-57.9%), and 28 ILs with low oil content (42.4-49.9%) were selected that may be released for cultivation upon further evaluation.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Peanut; Oil content; Oleic acid; Oil quality; Marker-assisted backcrossing; Marker-assisted selection; Groundnut
Subjects: Mandate crops > Groundnut
Depositing User: Mr Ramesh K
Date Deposited: 07 Sep 2015 05:42
Last Modified: 21 Oct 2016 08:33
URI: http://oar.icrisat.org/id/eprint/8986
Official URL: http://dx.doi.org/10.1016/j.plantsci.2015.08.013
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: Authors are thankful to Bryan Moss, K. Nagaraju and V. Papaiah for extending their technical assistance. The work presented in this article is contribution from research projects sponsored by Integrated Scheme of Oilseeds, Pulses, Oilpalm and Maize (ISOPOM), Department of Agriculture and Co-operation (DAC), Ministry of Agriculture, Government of India and MARS Chocolate North America, USA. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium.
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