Studies on fast and slow growing Rhizobium spp. nodulating Cajanus cajan and Cicer arietinum

Bromfield, E S P and Rao, J V D K K (1983) Studies on fast and slow growing Rhizobium spp. nodulating Cajanus cajan and Cicer arietinum. Annals of Applied Biology, 102 (3). pp. 485-493. ISSN 1744-7348

PDF - Published Version Restricted to ICRISAT users only Download (602kB) | Request a copy

Abstract

Fast and slow growing Rhizobium spp. isolated from Cajanus cajan and Cicer arietinum were compared in terms of colony characteristics, utilisation of carbon sources, acid production, symbiotic effectiveness and nodulating competitiveness. Fast growing isolates from C. cajan and C. arietinum formed 3–6 mm diameter colonies on yeast-extract mannitol agar after 4 days and were unlike the slow growers which produced colonies of c. 1 mm diameter after 7–10 days at 28 °C. The fast growing Rhizobium spp. from C. cajan utilised a wider range of carbon sources than the slow growing isolates from this host. Fast and slow growing strains from C. arietinum were able to utilise most of the carbon sources tested suggesting that the slow growers possessed glycolytic pathways similar to those in other fast growing species of Rhizobium. In culture, slow growing isolates from C. cajan produced a near-neutral to alkaline reaction (pH 66·7-5) whereas the fast growers from this host and both fast and slow growing isolates from C. arietinum produced an acidic reaction (pH 4·4–5·6). These data are discussed in the context of Norris' (1965) evolutionary concept of the Leguminosae. Under glassshouse conditions, fast and slow growing strains isolated from C. cajan and C. arietinum were equally effective on their respective hosts. In competition with slow growing rhizobia, half of the fast growers formed more than 70% of the nodules on C. cajan grown in sand. In all but one instance similar results were obtained when plants were grown in soil. With C. arietinum grown in sand or soil, all fast growing isolates from this host formed more than 85% of the nodules in competition with slow growing strains.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Pigeonpea
Depositing User:	Mr B K Murthy
Date Deposited:	30 Jul 2015 06:40
Last Modified:	30 Jul 2015 06:40
URI:	http://oar.icrisat.org/id/eprint/8878
Official URL:	http://dx.doi.org/10.1111/j.1744-7348.1983.tb02719...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

View Statistics

Actions (login required)

View Item