Development of NILs from heterogeneous inbred families for validating the rust resistance QTL in peanut (Arachis hypogaea L.)

    Yeri, S B and Shirasawa, K and Pandey, M K and Gowda, M V C and Sujay, V and Shriswathi, M and Nadaf, H L and Motagi, B N and Lingaraju, S and Lingaraju, A R S and Varshney, R K and Krishnaraj, P U and Bhat, R S (2014) Development of NILs from heterogeneous inbred families for validating the rust resistance QTL in peanut (Arachis hypogaea L.). Plant Breeding, 133 (1). pp. 80-85. ISSN 0179-9541

    [img] PDF - Published Version
    Restricted to ICRISAT users only
    Download (365kB) | Request a copy

    Abstract

    Heterogeneous inbred families segregating for rust resistance were identified from the two crosses involving susceptible (TAG 24 and TG 26) and resistant (GPBD 4) varieties of peanut. Rust-resistant (less than score 5) and rust-susceptible (more than score 5) plants were identified in each HIF and evaluated under rust epiphytotic conditions. The set of plants belonging to the same HIF, but differing significantly in rust resistance, not in other morphological and productivity traits, was regarded as near-isogenic lines (NILs). Largely, rust-resistant NILs had GPBD 4-type allele, and susceptible NILs carried either TAG 24 or TG 26-type allele at the three SSR loci (IPAHM103, GM1536 and GM2301) linked to a major genomic region governing rust resistance. Comparison of the remaining genomic regions between the NILs originating from each of the HIFs using transposon markers indicated a considerably high similarity of 86.4% and 83.1% in TAG 24 × GPPBD 4 and TG 26 × GPBD 4, respectively. These NILs are useful for fine mapping and expression analysis of rust resistance.

    Item Type: Article
    Divisions: RP-Grain Legumes
    CRP: CGIAR Research Program on Grain Legumes
    Uncontrolled Keywords: Background Markers; Genome Similarity; Heterogeneous Inbred Families; Near-isogenic lines; Peanut; Rust Resistance; Rust-resistance-linked markers
    Subjects: Mandate crops > Groundnut
    Depositing User: Mr Ramesh K
    Date Deposited: 17 Jul 2015 09:31
    Last Modified: 01 Mar 2016 09:09
    URI: http://oar.icrisat.org/id/eprint/8875
    Official URL: http://dx.doi.org/10.1111/pbr.12130
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
    Links:
    View Statistics

    Actions (login required)

    View Item View Item
    OAR@ICRISAT is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.