Isolation and characterization of a geminivirus causing yellow mosaic disease of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) in India.

    Muniyappa, V and Rajeshwari, R and Bharathan, N and Reddy, D V R and Nolt, B L (1987) Isolation and characterization of a geminivirus causing yellow mosaic disease of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) in India. Journal of Phytopathology, 119 (1). pp. 81-87. ISSN 0931-1785

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    Abstract

    Horsegram yellow mosaic disease was shown to be caused by a geminivirus; horsegram yellow mosaic virus (HYMV). The virus could not be transmitted by mechanical sap inoculation. Leaf dip and purified virus preparations showed geminate virus particles, measuring 15-18 * 30 nm. An antiserum for HYMV was produced and in enzyme-linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) tests HYMV was detected in leaf extracts of fieldinfected bambara groundnut, french bean, groundnut, limabean, mungbean, pigeonpea and soybean showing yellow mosaic symptoms. Bemisia tabaci fed on purified HYMV through a parafilm membrane transmitted the virus to all the hosts listed above but not to Ageratum conyzoides, okra, cassava, cowpea, Croton bonplandianus, Lab-lab purpureus, Malvastrum coromandalianum and tomato. No reaction was obtained in ELISA and ISEM tests between HYMV antibodies and extracts of plants diseased by whitefly-transmitted agents in India such as A. conyzoides yellow mosaic, okra yellow vein mosaic, C. bonplandianus, yellow vein mosaic, M. coromandalianum yellow vein mosaic, tomato leaf curl and cassava mosaic. HYMV was also not found to be related serologically to bean golden mosaic, virus.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Others > Plant Pathology
    Others > Entomology
    Depositing User: Mr B K Murthy
    Date Deposited: 03 Jul 2015 10:03
    Last Modified: 03 Jul 2015 10:03
    URI: http://oar.icrisat.org/id/eprint/8825
    Official URL: http://dx.doi.org/10.1111/j.1439-0434.1987.tb04386...
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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