Marker-trait association study for protein content in chickpea (Cicer arietinum L.)

Jadhav, A A and Rayate, S J and Mhase, L B and Thudi, M and Chitikineni, A and Harer, P N and Jadhav, A S and Varshney, R K and Kulwal, P L (2015) Marker-trait association study for protein content in chickpea (Cicer arietinum L.). Journal of Genetics. ISSN 0973-7731

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Abstract

Chickpea (Cicer arietinum L.) is the second most important cool season food legume cultivated in arid and semiarid regions of the world. The objective of the present study was to study variation for protein content in chickpea germplasm, and to find markers associated with it. A set of 187 genotypes comprising both international and exotic collections, and representing both desi and kabuli types with protein content ranging from 13.25% to 26.77% was used. Twenty-three SSR markers representing all eight linkage groups (LG) amplifying 153 loci were used for the analysis. Population structure analysis identified three subpopulations, and corresponding Q values of principal components were used to take care of population structure in the analysis which was performed using general linear and mixed linear models. Marker-trait association (MTA) analysis identified nine significant associations representing four QTLs in the entire population. Subpopulation analyses identified ten significant MTAs representing five QTLs, four of which were common with that of the entire population. Two most significant QTLs linked with markers TR26.205 and CaM1068.195 were present on LG3 and LG5. Gene ontology search identified 29 candidate genes in the region of significant MTAs on LG3. The present study will be helpful in concentrating on LG3 and LG5 for identification of closely linked markers for protein content in chickpea and for their use in molecular breeding programme for nutritional quality improvement.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Chickpea; Protein Content; Association Analysis; Population Structure; Marker-trait Association; Quantitative trait Locus
Subjects: Mandate crops > Chickpea
Depositing User: Mr Ramesh K
Date Deposited: 22 Jun 2015 06:36
Last Modified: 21 Oct 2016 06:14
URI: http://oar.icrisat.org/id/eprint/8805
Official URL: http://dx.doi.org/10.1007/s12041-015-0529-6
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: Thanks are due to the Head, Department of Agricultural Process Engineering and For age Breeding Scheme, MPAU, Rahuri, for providing the facilities for estimation of protein content. Thanks are also due to Aamir W. Khan, ICRISAT, for useful discussion on identifying candidate genes in the QTL region.
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