Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors

Nagaraju, M and Reddy, P S and Anil Kumar, S and Srivastava, R K and Kavi Kishor, P B and Rao, D M (2015) Genome-wide Scanning and Characterization of Sorghum bicolor L. Heat Shock Transcription Factors. Current Genomics, 16 (4). pp. 279-291. ISSN 1875-5488

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Abstract

A genome-wide scanning of Sorghum bicolor resulted in the identification of 25 SbHsf genes. Phylogenetic analysis shows the ortholog genes that are clustered with only rice, representing a common ancestor. Promoter analysis revealed the identification of different cis-acting elements that are responsible for abiotic as well as biotic stresses. Hsf domains like DBD, NLS, NES, and AHA have been analyzed for their sequence similarity and functional characterization. Tissue specific expression patterns of Hsfs in different tissues like mature embryo, seedling, root, and panicle were studied using real-time PCR. While Hsfs4 and 22 are highly expressed in panicle, 4 and 9 are expressed in seedlings. Sorghum plants were exposed to different abiotic stress treatments but no expression of any Hsf was observed when seedlings were treated with ABA. High level expression of Hsf1 was noticed during high temperature as well as cold stresses, 4 and 6 during salt and 5, 6, 10, 13, 19, 23 and 25 during drought stress. This comprehensive analysis of SbHsf genes will provide an insight on how these genes are regulated in different tissues and also under different abiotic stresses and help to determine the functions of Hsfs during drought and temperature stress tolerance.

Item Type: Article
Divisions: RP-Dryland Cereals
CRPS: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Heat shock transcription factors, Phylogenetic analysis, Cis-acting elements, Paralogs, Molecular chaperones, Abiotic stress
Subjects: Mandate crops > Sorghum
Others > Genetics and Genomics
Depositing User: Mr Ramesh K
Date Deposited: 15 May 2015 04:11
Last Modified: 21 Apr 2016 11:00
URI: http://oar.icrisat.org/id/eprint/8725
Official URL: http://benthamscience.com/journal/abstracts.php?jo...
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: MN is thankful to the UGC, New Delhi, for providing fellowship. PSR is thankful to the Department of Science and Technology, Govt. of India, New Delhi, for research funding through the INSPIRE Faculty Award Grant No. IFA-11LSPA-06. PBK is thankful to the CSIR, New Delhi, for providing Emeritus Fellowship. DMR is thankful to the UGC, New Delhi, for financial support through UGCMRP.
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