Bayer, P E and Ruperao, P and Mason, A S and Stiller, J and Chan, C K K and Hayashi, S and Long, Y and Meng, J and Sutton, T and Visendi, P and Varshney, R K and Batley, J and Edwards, D (2015) High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus. Theoretical and Applied Genetics, 128 (6). pp. 1039-1047. ISSN 0040-1706
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Abstract
The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
| Item Type: | Article |
|---|---|
| Divisions: | RP-Grain Legumes |
| CRP: | CGIAR Research Program on Grain Legumes |
| Subjects: | Others > Genetics and Genomics |
| Depositing User: | Mr B K Murthy |
| Date Deposited: | 16 Apr 2015 05:26 |
| Last Modified: | 05 Feb 2018 01:15 |
| URI: | http://oar.icrisat.org/id/eprint/8659 |
| Official URL: | http://dx.doi.org/10.1007/s00122-015-2488-y |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
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