First products of DNA marker-assisted selection in sorghum released for cultivation by farmers in sub-saharan Africa

Mohamed, A and Ali, R and Elhassan, O and Suliman, E and Mugoya, C and Masiga, C W and Elhusien, A and Hash, C T (2014) First products of DNA marker-assisted selection in sorghum released for cultivation by farmers in sub-saharan Africa. Journal of Plant Science & Molecular Breeding, 3 (3). pp. 1-10. ISSN 2050-2389

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Abstract

Striga hermonthica (Del.) Benth. is the major biotic constraint to sorghum production. Its control is difficult and can only be achieved through integrated management strategies that depend mainly on host plant resistance and enhanced soil fertility. However, breeding for resistance is hampered by the complexity of host parasite interactions and lack of reliable screening methods. The invention of molecular markers has enhanced the effectiveness of breeding for resistance. Five genomic regions (QTLs) with linked markers associated with Striga resistance were mapped in sorghum variety N13 by [10]. In this study, to increase the efficiency of marker-assisted selection (MAS), 27 EST-SSR markers in close association with Striga resistance QTLs were also identified and mapped. Populations of backcross (BC3S4) derived from N13 (Striga resistant) X three farmer preferred sorghum cultivars: Tabat, Wad Ahmed and AG-8 (Striga susceptible) were generated. Thirty-one lines (BC3S4) with confirmed Striga field resistance were genotyped with foreground and background selection makers. Twenty resistant lines, with two or more major QTLs were selected for regional evaluation. Of these 10 lines were selected and advanced for multi-location testing, together with Wad Ahmed, Tabat, AG-8, N13, SRN39 and IS9830 as checks. Standard variety trials were conducted in Striga sick plots over three seasons (2009-2011) in Sudan, Gezira Research Station, Damazine, Sinnar, and Gedarif. Results revealed that four lines (T1BC3S4, AG6BC3S4, AG2BC3S4 and W2BC3S4) were Striga resistant and agronomically superior with yields ranging from 180% to 298% higher relative to their recurrent parents. This Striga resistance coupled with superior attributes of the recurrent parent (including very high yield potentials, high grain quality and drought tolerance) will provide adaptation and stability across a wide range of environments. These are the first products of DNA markerassisted selection (MAS) in sorghum released for cultivation by farmers in sub-Saharan Africa.

Item Type: Article
Divisions: RP-Dryland Cereals
CRPS: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Striga hermonthica, Molecular markers, Quantitative trait loci (QTL), Simple sequence repeat (SSR)
Subjects: Mandate crops > Sorghum
Depositing User: Mr B K Murthy
Date Deposited: 08 Apr 2015 07:53
Last Modified: 01 Mar 2016 09:39
URI: http://oar.icrisat.org/id/eprint/8647
Official URL: http://dx.doi.org/10.7243/2050-2389-3-3
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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