Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana)

De Villiers, S M and Michael, V N and Manyasa, E O and Saiyiorri, A N and Deshpande, S (2015) Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana). Electronic Journal of Biotechnology, 18 (2). pp. 77-82. ISSN 0717-3458

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Abstract

Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3) polymorphism and ability to discern genetic diversity within the tested finger millet germplasm.

Item Type: Article
Divisions: RP-Dryland Cereals
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Diversity analysis; SSR markers; Genotyping ; East Africa, Finger millet
Subjects: Mandate crops > Millets > Finger Millet
Depositing User: Mr Ramesh K
Date Deposited: 10 Mar 2015 11:09
Last Modified: 20 Oct 2016 05:14
URI: http://oar.icrisat.org/id/eprint/8594
Official URL: http://dx.doi.org/10.1016/j.ejbt.2014.12.001
Projects: UNSPECIFIED
Funders: Open Access funded by Pontificia Universidad Católica de Valparaíso
Acknowledgement: UNSPECIFIED
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