Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.]

Ajay, B C and Gowda, M B and Prasad, P S and Veerakumar, G N and Babu, P H and Ganesh, B N and Venkatesh, S C and Ganapathy, K N and Fiyaz, R A and Ramya, K T (2015) Identification of AFLP markers linked to Fusarium wilt disease in pigeonpea [Cajanus cajan (L.) Millsp.]. Legume Research, 38 (1). pp. 126-130. ISSN 0250-5371

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Abstract

An experiment was conducted to identify markers linked to Fusarium wilt disease resistance, Parents namely TTB 7 and ICP 8863 were screened using 151 SSRs markers and 16 AFLP primer combinations. Parental screening revealed five SSR primers and 12 AFLP primer combinations polymorphic between parents. Bulk segregant analysis identified five AFLP primer combinations generating seven markers polymorphic between resistant and susceptible bulks while, none of the SSR markers were polymorphic. This indicates that, these markers are putatively linked to wilt disease. Screening of F2 segregating population of cross TTB 7 x ICP 8863 with these putatively linked markers revealed four markers (E-AAT/M-CTG850, ETCG/M-CTT650, E-TCG/M-CTA730 and E-TCG/M-CTT230) which segregated in 3:1 mendelian pattern. Simple linear regression performed on these four markers had identified two markers namely E-TCG/M-CTT650 and E-TCG/M-CTA730 linked to disease.

Item Type: Article
Divisions: RP-Grain Legumes
CRPS: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: AFLP, BSA, Fusarium wilt disease, Linkage, Pigeonpea
Subjects: Mandate crops > Pigeonpea
Others > Entomology
Depositing User: Mr B K Murthy
Date Deposited: 10 Mar 2015 10:31
Last Modified: 10 Mar 2015 10:31
URI: http://oar.icrisat.org/id/eprint/8589
Official URL:
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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