Detection of aflatoxigenic Aspergillus strains by cultural and molecular methods: A critical review

Sudini, H and Srilakshmi, P and Vijay Krishna Kumar, K and Njoroge, S M C and Osiru, M and Seetha, A and Waliyar, F (2015) Detection of aflatoxigenic Aspergillus strains by cultural and molecular methods: A critical review. African Journal of Microbiology Research, 9 (8). pp. 484-491. ISSN 1996-0808

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Abstract

Aflatoxin contamination of food and feed commodities, caused by Aspergillus section Flavi group of fungi, is a serious problem worldwide. Exposure through consumption of contaminated food and feed has deleterious effects on human and animal health. Therefore, aflatoxin contaminated products are a barrier to international trade of agricultural commodities. Not all fungi from Aspergillus section Flavi produce aflatoxins. Hence it is important to differentiate Aspergillus spp. into toxigenic and atoxigenic species to better understand their population structure in a specific environment. A range of methods are available today, including cultural, analytical and molecular methods, to identify the toxin producing ability of isolates from section Flavi. A comprehensive review of these methods would be of great use for researchers in developing nations where fully equipped aflatoxin detection laboratories are lacking. In this paper we critically reviewed the cultural and molecular methods of detecting aflatoxigenic Aspergillus species and their precision.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Toxigenic Aspergillus, atoxigenic Aspergillus, cultural methods, PCR based methods
Subjects: Others > Food legumes
Others > Plant Pathology
Others > Food and Nutrition
Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Mr Ramesh K
Date Deposited: 24 Feb 2015 08:37
Last Modified: 21 Oct 2016 05:43
URI: http://oar.icrisat.org/id/eprint/8570
Official URL: http://dx.doi.org/10.5897/AJMR2014.7309
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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