DREB1A overexpression in transgenic chickpea alters key traits influencing plant water budget across water regimes

Anbazhagan, K and Bhatnagar-Mathur, P and Vadez, V and Reddy, D S and Kishor, P B K and Sharma, K K (2015) DREB1A overexpression in transgenic chickpea alters key traits influencing plant water budget across water regimes. Plant Cell Reports, 34 (2). pp. 199-210. ISSN 0721-7714

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Abstract

Chickpea (Cicer arietinum L.) is mostly exposed to terminal drought stress which adversely influences its yield. Development of cultivars for suitable drought environments can offer sustainable solutions. We genetically engineered a desi-type chickpea variety to ectopically overexpress AtDREB1A, a transcription factor known to be involved in abiotic stress response, driven by the stress-inducible Atrd29A promoter. From several transgenic events of chickpea developed by Agrobacterium-mediated genetic transformation, four single copy events (RD2, RD7, RD9 and RD10) were characterized for DREB1A gene overexpression and evaluated under water stress in a biosafety greenhouse at T6 generation. Under progressive water stress, all transgenic events showed increased DREB1A gene expression before 50 % of soil moisture was lost (50 % FTSW or fraction of transpirable soil water), with a faster DREB1A transcript accumulation in RD2 at 85 % FTSW. Compared to the untransformed control, RD2 reduced its transpiration in drier soil and higher vapor pressure deficit (VPD) range (2.0–3.4 kPa). The assessment of terminal water stress response using lysimetric system that closely mimics the soil conditions in the field, showed that transgenic events RD7 and RD10 had increased biomass partitioning into shoot, denser rooting in deeper layers of soil profile and higher transpiration efficiency than the untransformed control. Also, RD9 with deeper roots and RD10 with higher root diameter showed that the transgenic events had altered rooting pattern compared to the untransformed control. These results indicate the implicit influence of rd29A::DREB1A on mechanisms underlying water uptake, stomatal response, transpiration efficiency and rooting architecture in water-stressed plants.

Item Type: Article
Divisions: RP-Dryland Cereals
CRP: CGIAR Research Program on Grain Legumes
Uncontrolled Keywords: Chickpea, DREB1A, Drought, Root length density, Transpiration efficiency, Vapor pressure deficit
Subjects: Mandate crops > Chickpea
Others > Genetics and Genomics
Others > Plant Physiology
Depositing User: Mr B K Murthy
Date Deposited: 23 Jan 2015 04:34
Last Modified: 23 Jul 2018 05:26
URI: http://oar.icrisat.org/id/eprint/8488
Official URL: http://dx.doi.org/10.1007/s00299-014-1699-z
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This work was supported by funds from the Indo-Swiss Collaboration for Biotechnology (ISCB) that was jointly funded by the Swiss Agency for Development and Cooperation (SDC), Switzerland and the Department of Biotechnology (DBT), Government of India. We are grateful to Dr. K. Yamaguchi-Shinozaki, Japan International Research Center for Agricultural Sciences (JIRCAS), Japan, for providing the gene construct used for developing transgenic events. KA would like to acknowledge financial support from the Council for Scientific and Industrial Research (CSIR), Government of India, for her Ph.D. Program. This work was undertaken as part of the CGIAR Research Program on Grain Legumes.
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