Genetic engineering of soybean trypsin inhibitor gene in groundnut (Arachis hypogaea L.) for induced resistance to insect pests

Shashi Rekha, T (1999) Genetic engineering of soybean trypsin inhibitor gene in groundnut (Arachis hypogaea L.) for induced resistance to insect pests. Masters thesis, University of Calicut.

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Abstract

By using various concentrations of auxins and cytokinins it was found that 3 mg/L BAP+lmg/L NAA is ideal for regeneration of the leaflet explants of groundnut.Transformation of the leaflet explants of groundnut with soybean trypsin inhibitor (SBTI) gene had been done through Agrobacteriwn.The leaflet explants of groundnut had also been transformed with SBTI gene through Biolistics.The putatively transformed leaflet explants were assayed histochemically for the presence of this gene in the transformants.

Item Type:	Thesis (Masters)
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Groundnut
Depositing User:	Users 6 not found.
Date Deposited:	02 Sep 2011 10:14
Last Modified:	02 Sep 2011 10:14
URI:	http://oar.icrisat.org/id/eprint/832
Acknowledgement:	UNSPECIFIED
Links:	Author

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