Comparison of different methods for extraction and estimation of aflatoxin B1 in groundnut

Mehan, V K and Bhavanishankar, T N and Bedi, J S (1985) Comparison of different methods for extraction and estimation of aflatoxin B1 in groundnut. Journal of Food Science and Technology, 22 (2). pp. 123-125. ISSN 0022-1155

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Abstract

Aflatoxin extraction methods namely Best Food (BF), Contaminant Branch (CB), Pous’ and Romer’s and analytical methods namely thin-layer chromatography, spectrophotometric and minicolumn techniques, in different combinations, were compared for their efficiency for determining aflatoxin B1 occurring naturally in groundnuts and also for estimating aflatoxin B1 levels in groundnut meal samples spiked with known quantity of the toxin. The BF and Pons’ methods gave better efficiency than the other two methods. Spectrophotometric method was more effective than TLC and minicolumn techniques. BF method was found relatively less expensive and less time consuming as compared to other extraction methods. The Pons’ method was found convenient while handling large numbers of samples especially in the absence of centrifuge facilities required for the BF method.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Aflatoxin B1
Subjects:	Mandate crops > Groundnut Others > Entomology
Depositing User:	Mr B K Murthy
Date Deposited:	30 Jul 2014 10:45
Last Modified:	30 Jul 2014 10:45
URI:	http://oar.icrisat.org/id/eprint/8252
Official URL:
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

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