Crop hosts and genotypic resistance influence the biological activity of Bacillus thuringiensis towards Helicoverpa armigera

    Paramasiva, I and Krishnayya, P V and War, A R and Sharma, H C (2014) Crop hosts and genotypic resistance influence the biological activity of Bacillus thuringiensis towards Helicoverpa armigera. Crop Protection, 64. pp. 38-46. ISSN 0261-2194

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    Abstract

    Studies on the influence of genotypic resistance on biological activity of a commercial formulation of Bacillus thuringiensis (Bt) and pure Bt toxin Cry1Ac were carried out to develop appropriate strategies for pod borer, Helicoverpa armigera management in chickpea, sorghum, pigeonpea and cotton. The interaction effects of host plant resistance and biological activity of commercial Bt/Cry1Ac were studied by incorporating the lyophilized tissues of chickpea leaves, milk stage sorghum grain, pigeonpea pods and cotton squares into the artificial diet with and without Bt formulation or Cry1Ac. The H. armigera larval weights were significantly lower in insects reared on diets with square powder of the insect - resistant Bt-cotton RCH 2 + Bt/Cry1Ac and pod powder of insect - resistant pigeonpea genotype, ICPL 332WR + Bt/Cry1Ac as compared to the larvae reared on diets with leaf powder of H. armigera susceptible chickpea genotype, ICCC 37 and the standard artificial diet. Pupation and adult emergence were significantly lower in insects reared on diets with tissues of pod borer-resistant genotypes + Bt/Cry1Ac as compared to insects reared on diets with tissues of the insect susceptible genotypes + Bt/Cry1Ac. Insects reared on diets containing insect-resistant and -susceptible genotypes of sorghum, pigeonpea and cotton and pod borer-resistant genotype of chickpea (ICC 506EB) + Bt/Cry1Ac did not lay any eggs. However, eggs were laid by the insects reared on diets containing pod borer-susceptible genotype of chickpea, ICCC 37 and on the standard artificial diet + Bt/Cry1Ac. The insects reared on diets with sorghum genotype, ICSV 745, and Bt-cotton, RCH 2 without Bt/Cry1Ac also did not lay eggs. The results suggested that Bt/Cry1Ac is more effective for management of H. armigera when deployed in combination with insect-resistant genotypes of cotton, chickpea, pigeonpea and sorghum.

    Item Type: Article
    Divisions: RP-Grain Legumes
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Genotypic resistance; Bacillus thuringiensis; Helicoverpa armigera; Cry1Ac; Biological activity; Pest management
    Subjects: Others > Entomology
    Depositing User: Mr Siva Shankar
    Date Deposited: 09 Jul 2014 05:12
    Last Modified: 09 Jul 2014 06:18
    URI: http://oar.icrisat.org/id/eprint/8188
    Official URL: http://dx.doi.org/10.1016/j.cropro.2014.05.010
    Projects: UNSPECIFIED
    Funders: Government of India - Department of Agriculture and Cooperation
    Acknowledgement: UNSPECIFIED
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