Alcohol dehydrogenase and invertase activities in ethanol tolerant yeasts

Gokhale, D V and Rao , B S and Sivaramakrishnan, S (1986) Alcohol dehydrogenase and invertase activities in ethanol tolerant yeasts. Enzyme and Microbial Technology, 8 (10). pp. 623-626. ISSN 0141-0229

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Abstract

Two ethanol tolerant yeast isolates of Saccharomyces cerevisiae Y-10 and Saccharomyces cerevisiae Y-7 were compared for their invertase (EC 3.2.1.26, β-fructofuranoside fructohydrolase) and alcohol dehydrogenase (ADH, EC 1.1.1.1, alcohol:NAD oxidoreductase) activities as well as ethanol productivity. The isolates showed significantly higher ethanol productivities compared to the standard strain Saccharomyces uvarum and other yeast strains tested. The alcohol dehydrogenase activity was 40–100% higher in the isolates than S. uvarum and the percentage change varied depending on the growth conditions. However, this was not true for invertase activity. Both the isolates showed a similar ADH isozyme pattern in contrast to S. uvarum. The results suggested that a better correlation between ADH activity and ethanol productivity could be drawn only after extensive studies on the kinetic parameters of the individual isozymes.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Ethanol productivity; molasses fermentation; alcohol dehydrogenase (ADH); isozyme pattern
Subjects: Others > Fertilizer Applications
Others > Entomology
Depositing User: Mr B K Murthy
Date Deposited: 15 Jul 2014 06:12
Last Modified: 15 Jul 2014 06:12
URI: http://oar.icrisat.org/id/eprint/8180
Official URL: http://dx.doi.org/10.1016/0141-0229(86)90123-7
Projects: Government of India DP/IND/80/003
Funders: UNDP
Acknowledgement: UNSPECIFIED
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