Simultaneous detection of groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and satellite RNA (satRNA) in groundnuts using multiplex RT-PCR

Anitha, S and Monyo, E S and Okori, P (2014) Simultaneous detection of groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and satellite RNA (satRNA) in groundnuts using multiplex RT-PCR. Archives of Virology. pp. 1-4. ISSN 0304-8608

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Abstract

Groundnut rosette disease (GRD) is the most devastating disease of groundnuts in sub-Saharan Africa. The disease is caused by synergistic interactions between viruses and virus-like pathogens: groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and a satellite RNA (satRNA). The multi-pathogenic nature of GRD requires efficient diagnostic systems for plant breeding and pathology work. Currently, TAS-ELISA and RT-PCR are used to detect all three pathogens. This approach is time-consuming, expensive and not easily amenable to high throughput. A multiplex PCR-based approach was developed to detect all three pathogens at once, reducing diagnostics costs and time by two thirds. The technique is highly robust and amenable to high throughput, with sensitivity and specificity values of 88 % and 100 %, respectively. The positive predictive value for the technique is 100 %, and the negative predictive value is 90.6 %.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Grain Legumes
Subjects: Mandate crops > Groundnut
Others > Plant Virology
Depositing User: Mr Siva Shankar
Date Deposited: 20 Jun 2014 08:02
Last Modified: 21 Feb 2018 08:06
URI: http://oar.icrisat.org/id/eprint/8141
Official URL: http://dx.doi.org/10.1007/s00705-014-2139-7
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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