Mitochondrial P-Glycoprotein ATPase Contributes to Insecticide Resistance in the Cotton Bollworm, Helicoverpa armigera (Noctuidae: Lepidoptera)

Akbar, S Md and Aurade, R M and Sharma, H C and Sreeramulu, K (2014) Mitochondrial P-Glycoprotein ATPase Contributes to Insecticide Resistance in the Cotton Bollworm, Helicoverpa armigera (Noctuidae: Lepidoptera). Cell Biochemistry and Biophysics. ISSN 1559-0283

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Abstract

Cotton bollworm, Helicoverpa armigera, is one of the most damaging polyphagous pests worldwide, which has developed high levels of resistance to commonly applied insecticides. Mitochondrial P-glycoprotein (Pgp) was detected in the insecticide-resistant strain of H. armigera using C219 antibodies, and its possible role was demonstrated in the efflux of xenobiotic compounds using spectrofluorometer. The TMR accumulated in mitochondria in the absence of ATP, and effluxed out in presence of ATP; the process of efflux was inhibited in the presence of ortho-vandate, an inhibitor of Pgp, in insecticide-resistant larvae of H. armigera. The mitochondria isolated from insecticide-resistant larvae were resistant to insecticide-induced inhibition of oxygen consumption and cytochrome c release. Membrane potential decreased in a dose-dependent manner in the presence of higher concentration of insecticides (>50 µM) in mitochondria of insecticide-resistant larvae. In conclusion, mitochondrial Pgp ATPase detected in the insecticide-resistant larvae influenced the efflux of xenobiotic compounds. Pgp might be involved in protecting the mitochondrial DNA and the components of the electron transport chain from damage due to insecticides, and contributing to the resistance to the deleterious effects of insecticides on the growth of insecticide-resistant H. armigera larvae.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: UNSPECIFIED
Uncontrolled Keywords: Helicoverpa armigera, Mitochondria, Multidrug resistance, Pgp ATPase, Insecticides,
Subjects: Others > Entomology
Depositing User: Mr Siva Shankar
Date Deposited: 26 Apr 2014 07:49
Last Modified: 06 Aug 2015 10:36
URI: http://oar.icrisat.org/id/eprint/7907
Official URL: http://dx.doi.org/10.1007/s12013-014-9969-5
Projects: UNSPECIFIED
Funders: UGC - New Delhi, UGC – SAP (DSR - I), New Delhi, Ministry of Agriculture, Government of India
Acknowledgement: UNSPECIFIED
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