Identification of bud blight of soybean (Glycine max L. Merr.) through ELISA and infectivity assay

Thakur, M P and Reddy, D V R and Reddy, A S and Ratna, A S and Al-Nasiri, M and Agrawal , K C (1996) Identification of bud blight of soybean (Glycine max L. Merr.) through ELISA and infectivity assay. Indian Journal of Virology, 12 (1). pp. 79-82. ISSN 0970-2822

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Abstract

The virus from soyabean bud blight infected plants reacted strongly with the antisera of peanut bud necrosis tospovirus (BNV) using direct antigen coating-ELISA. Of 19 soyabean samples with bud blight symptoms, 14 were positive for BNV alone. One sample was positive for BNV, cowpea mild mottle carlavirus and peanut stripe virus. The results of dot immunobinding assay were positive with the antisera of BNV at 10-1 and 10-2 dilutions. Mechanical inoculation on cowpea cv. C-152 and groundnut cv. JL 24 resulted in necrotic ring spots and chlorotic spots, respectively, after 6 d. These symptoms were characteristic of BNV and it is suggested that BNV is associated with soyabean bud blight in India

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Subjects: Others > Entomology
Depositing User: Mr B K Murthy
Date Deposited: 16 Apr 2014 08:36
Last Modified: 16 Apr 2014 08:36
URI: http://oar.icrisat.org/id/eprint/7837
Official URL:
Projects: UNSPECIFIED
Funders: ICRISAT(Patancheru)
Acknowledgement: The first author is thankful to the Training and Fellowship Division, ICRISAT, Patancheru (A.P.) for financial assistance
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