Molecular analysis for genetic structure of biotic and abiotic stress resistant genotypes in chickpea (Cicer arietinum L.)

Singh, Reema and Kumari, N and Upadhyaya, H D and Yadav, R and Vaishali, . and Choi, I and Kumar, R (2013) Molecular analysis for genetic structure of biotic and abiotic stress resistant genotypes in chickpea (Cicer arietinum L.). Indian Journal of Biotechnology, 12 (4). pp. 537-540. ISSN 0975-0967

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Abstract

Molecular characterization of biotic (Ascochyta blight, Fusarium wilt & dry root rot) and abiotic (drought & salinity) resistant genotypes of chickpea was carried out using a specific set of 20 polymorphic STMS (sequence tagged microsatellite site) markers. The number of alleles ranged from 1 to 3 alleles per locus. The PIC (polymorphism information content) value ranged from 0.0 to 0.656 with an average of 0.268, indicating the considerable efficiency of markers for studying the polymorphism level. The primer GA-33 showed maximum PIC value (0.656), while the ten primers had the 0.0 value. The dendogram derived from the analysis had six clusters. Many pairs showed the maximum similarity (0.983), whereas accessions ICC-1392 and ICC-2065 showed the minimum similarity (0.900). The conservation of genotypes ICC-2580, ICC-1392 and ICC-2065 was recommended for their utilization in Middle-east Asia region chickpea improvement programme.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Chickpea, Microsatellite marker, Molecular diversity, PIC, STMS
Subjects: Mandate crops > Chickpea
Others > Genetics and Genomics
Depositing User: Mr B K Murthy
Date Deposited: 17 Mar 2014 10:39
Last Modified: 17 Mar 2014 10:39
URI: http://oar.icrisat.org/id/eprint/7626
Official URL: http://nopr.niscair.res.in/bitstream/123456789/262...
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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