Expression of drought responsive genes in pigeonpea and in silico comparison with soybean cDNA library

Deeplanaik, N and Kumaran, R C and Venkatarangaiah, K and Shivashankar, S K H and Doddamani, D and Telkar, S (2013) Expression of drought responsive genes in pigeonpea and in silico comparison with soybean cDNA library. Journal of Crop Science and Biotechnology , 16 (4). pp. 243-251. ISSN 2005-8276

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Abstract

Pigeonpea, a drought tolerant, semi-arid pulse crop has been investigated for the expression of differentially expressed genes (DEGs) under drought stress. The cDNA library of soybean leaf tissue retrieved from the Unigene database of the NCBI, were compared for in silico expression using IDEG6 web statistical tool. A list of 52 non-redundant DEGs consisting of 11 up-regulated and 41 down-regulated was obtained. Among these, more photosynthesis and light harvesting proteins were down-regulated in drought stress conditions. Pathways were assigned based on KEGG database, revealing 32 genes involved in 17 metabolic pathways. Homologous sequences of six up-regulated genes namely, ADF3, APB, ASR, DLP, LTP1, and UGE5 were then used for quantitative reverse transcription PCR (qRT-PCR) in pigeonpea. The qRT-PCR result revealed the significant up-regulation of dehydrin-like protein (DLP) (5.02 log2 fold) and down-regulation of acid phosphatase class B family protein (APB) (9.43 log2 fold) and non-specific lipid transfer protein 1-like (LTP1) (18.81 log2 fold) in pigeonpea water-stressed leaf sample compared to well-watered leaf samples. No significant difference was observed in the stressed root compared to the stressed pigeonpea leaf sample except that APB showed an up-regulation of 11.35 log2 fold change

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: differentially expressed genes, drought stress, pigeonpea,qRT-PCR
Subjects: Mandate crops > Pigeonpea
Depositing User: Mr B K Murthy
Date Deposited: 15 Mar 2014 14:59
Last Modified: 15 Mar 2014 14:59
URI: http://oar.icrisat.org/id/eprint/7611
Official URL: http://dx.doi.org/10.1007/s12892-013-0069-7
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: This work is supported by Kuvempu University and University Grants Commission, India by awarding a research fellowship to Nagaraja Deeplanaik. Authors are thankful to Networking Resource Centre in Biological Sciences, Madurai Kamaraj University, India for awarding the visiting fellowship.
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