Stable transformation of Xylella fastidiosa with small repW shuttle vector pUFR047

Anjaiah, V and Gabriel, D W (2006) Stable transformation of Xylella fastidiosa with small repW shuttle vector pUFR047. Current Science, 91 (3). pp. 344-347. ISSN 0011-3891

Preview

PDF (Open Access Journal Article) - Published Version Download (90kB) | Preview

Abstract

Xylella fastidiosa (Xf ) is a fastidious, xylem-inhabiting, Gram-negative bacterium that causes serious plant diseases in a wide range of plant species. The most serious diseases are Pierce’s Disease (PD) of grape and Citrus Variegated Chlorosis (CVC). Functional genomic analyses of Xf have been severely limited by lack of a stable replicative shuttle vector. Plamsid pUFR047, small, stable, wide host range, conjugative and repW shuttle vector have been successfully transferred into Xf strains by electroporation. The vector replicated in a stable manner for over thirty generations of growth in the absence of antibiotic selection in Xf strains.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Others > Plant Pathology Others > Plant Virology Others > Agriculture-Farming, Production, Technology, Economics
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	20 Feb 2014 09:22
Last Modified:	20 Feb 2014 09:22
URI:	http://oar.icrisat.org/id/eprint/7546
Official URL:	http://www.iisc.ernet.in/~currsci/aug102006/344.pd...
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

View Statistics

Actions (login required)

View Item