DNA Cloning and Sequencing

Kishore Babu, B and Sharma, A and Sudini, H (2013) DNA Cloning and Sequencing. In: Analyzing Microbes: Manual of Molecular Biology Techniques. Springer Protocols Handbooks . Springer Berlin Heidelberg, pp. 291-302. ISBN 978-3-642-34410-7

PDF - Published Version Restricted to ICRISAT users only Download (107kB) | Request a copy

Abstract

The plasmid DNA is cleaved with an enzyme and joined in vitro to foreign DNA; the resulting recombinant plasmids are then used to transform bacteria. The plasmid vectors must be carefully chosen and processed to minimize the effort required to identify and characterize recombinants. This chapter provides guidelines for preparation of DNA fragment for cloning, transformation into chemically competent host, and selection of positive clones. The write-up will also describe basic methods used in the cloning of PCR amplified rRNA gene into appropriate vector and followed by sequencing.

Item Type:	Book Section
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Series Name:	Springer Protocols Handbooks
Subjects:	Others > Genetics and Genomics
Depositing User:	Mr Siva Shankar
Date Deposited:	06 Feb 2014 05:49
Last Modified:	10 May 2016 08:40
URI:	http://oar.icrisat.org/id/eprint/7465
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

View Statistics

Actions (login required)

View Item