Vegetative compatibility, host range and pathogenicity of Verticillium dahliae isolates in Iran

Sanei, S J and Waliyar, F and Razavi, S I and Okhovvat, S M (2008) Vegetative compatibility, host range and pathogenicity of Verticillium dahliae isolates in Iran. International Journal of Plant Production, 2 (1). pp. 37-46.

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Verticillium wilt is an economically important disease which inflicts serious losses in potato, cotton, alfalfa, some vegetable crops and fruit trees and occasionally ornamentals. V. dahliae, infected cultivated species and weeds were collected from several areas in Iran during twelve years from 1993-2005 and studied for their vegetative compatibility, host range and pathogenicity. The pathogen was isolated from 27 species belonging to 24 genera and 15 families of plants but was most frequently isolated from Solanaceae, Cucurbitaceae, Oleaceae and Rosaceae hosts. The morphology of V. dahliae isolates on Czapecks agar and water agar media were different especially for micosclerotial appearance time (4-19 days), pigmented zone of colony (37.8-48.33 mm) and microsclerotial morphology (abundant, irregular and elongated shaped or more spherical and scattered). The ratio index of length/width of conidia ranged between 2.32 and 2.70 micrometer with an average of 2.43 + 0.11. Temperature influenced the radial growth ratio of the isolates and the growth response of V. dahliae isolates to temperature in vitro was quadratic. All isolates were categorized in three groups based on pathogenicity tests on differential test plants (cotton cv. Sahel and aubergine cv. local). A total of 548 V. dahliae isolates from different locations and hosts were assigned to vegetative compatibility groups (VCGs) using nitrate-nonutilizing (nit) mutants. A higher frequency of nit1/nit3 mutants (93%) were obtained compared to nitM (7%). Approximately 51.1% of the isolates were assigned to VCG4B, 25.9% to VCG2A and 23% to VCG1. The results demonstrated that V. dahliae isolates assigned to VCGs were closely associated with specific pathogenicity within the group/diverse.

Item Type: Article
Subjects: Others > Agriculture-Farming, Production, Technology, Economics
Depositing User: Library ICRISAT
Date Deposited: 07 Oct 2011 06:14
Last Modified: 07 Oct 2011 06:14
Official URL:
Acknowledgement: UNSPECIFIED
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